概述

  • 产品名称Anti-Vitronectin抗体[BV1]
    参阅全部 Vitronectin 一抗
  • 描述
    小鼠单克隆抗体[BV1] to Vitronectin
  • 特异性The antibody binds to soluble vitronectin as well as to membrane bound vitronectin.
  • 经测试应用适用于: IHC-P, WB, IP, ICC/IF, Purificationmore details
  • 种属反应性
    与反应: Human

性能

应用

Our Abpromise guarantee covers the use of ab7165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 19730772
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Purification Use at an assay dependent concentration.

靶标

  • 功能Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • 组织特异性Plasma.
  • 序列相似性Contains 4 hemopexin repeats.
    Contains 1 SMB (somatomedin-B) domain.
  • 结构域The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
  • 翻译后修饰Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.
  • 细胞定位Secreted, extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Complement S Protein antibody
    • Epibolin antibody
    • S Protein antibody
    • S-protein antibody
    • Serum Spreading Factor antibody
    • Serum-spreading factor antibody
    • Somatomedin B antibody
    • Somatomedin-B antibody
    • V75 antibody
    • Vitronectin V10 subunit antibody
    • Vitronectin V65 subunit antibody
    • VN antibody
    • VNT antibody
    • VTN antibody
    • VTNC_HUMAN antibody
    see all

Anti-Vitronectin antibody [BV1] (ab7165)参考文献

This product has been referenced in:
  • Knappe UJ  et al. Expression of extracellular matrix-proteins in perisellar connective tissue and dura mater. Acta Neurochir (Wien) 152:345-53 (2010). IHC-P ; Human . Read more (PubMed: 19730772) »

See 1 Publication for this product

Product Wall

We regret to hear about your problems with ab7165 monoclonal antibody to human vitronectin. The antibody is described in the following article: Zanetti, A et al; Clustering of vitronectin and RGD peptides on microspheres leads to engagement of inte...

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I have contacted the source of the antibody to find out if they can recommend a protocol. Could you please confirm you used protease inhibitors and kept the samples cold at all times during your experiment prior to boiling your samples with loading...

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I'm sorry you are having problems with ab7165 and would like to thank you for taking the time to give me details of your protocol, it is very useful. I would like to suggest incubating overnight at 4C and try also more concentrated antibody. Co...

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The subunit recognise by this antibody is undetermined, as is it's cross-reactivity with mouse.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"