Anti-Vitronectin抗体[A18] (ab23444)

概述

  • 产品名称Anti-Vitronectin抗体[A18]
    参阅全部 Vitronectin 一抗
  • 描述
    小鼠单克隆抗体[A18] to Vitronectin
  • 特异性ab23444 is highly specific for Vitronectin. There is no evidence for cross reactivity with other connective tissue proteins (Fibronectin, Elastin, Collagen, Laminin).
  • 经测试应用适用于: IHC-P, Inhibition Assay, ELISA, IHC-Frmore details
    不适用于: WB
  • 种属反应性
    与反应: Rabbit, Horse, Cow
    不与反应: Sheep, Human
  • 免疫原

    Tissue/ cell preparation (Cow): Lysed bovine corneal endothelial cells and extracellular matrix

  • 常规说明

    ab23444 can be used to probe vitronectin conformation.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 500mM Sodium chloride, 10mM PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号A18
  • 骨髓瘤Sp2/0
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab23444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.

ab23444 inhibits integrin mediated cell adhesion to bovine vitronectin.

ELISA 1/500.
IHC-Fr 1/500.

1/500 for PLP fixed samples.

  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
      Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
    • 组织特异性Plasma.
    • 序列相似性Contains 4 hemopexin repeats.
      Contains 1 SMB (somatomedin-B) domain.
    • 结构域The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
    • 翻译后修饰Sulfated on 2 tyrosine residues.
      N- and O-glycosylated.
      Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
      It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
      Phosphorylation sites are present in the extracellular medium.
    • 细胞定位Secreted, extracellular space.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Complement S Protein antibody
      • Epibolin antibody
      • S Protein antibody
      • S-protein antibody
      • Serum Spreading Factor antibody
      • Serum-spreading factor antibody
      • Somatomedin B antibody
      • Somatomedin-B antibody
      • V75 antibody
      • Vitronectin V10 subunit antibody
      • Vitronectin V65 subunit antibody
      • VN antibody
      • VNT antibody
      • VTN antibody
      • VTNC_HUMAN antibody
      see all

    Anti-Vitronectin antibody [A18] (ab23444)参考文献

    ab23444 has not yet been referenced specifically in any publications.

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