概述

  • 产品名称
    Anti-Vitellogenin抗体[JE-10D4]
    参阅全部 Vitellogenin 一抗
  • 描述
    小鼠单克隆抗体[JE-10D4] to Vitellogenin
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Zebrafish
  • 免疫原

    Full length native vitellogenin purified from whole body homogenate of 17 beta estradiol treated zebrafish.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 12.3mM Sodium Azide
    Constituents: 1% BSA, 0.15M Sodium chloride, 0.1M Sodium phosphate, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    JE-10D4
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab36804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明
    ELISA: 1/100 - 1/1000.
    Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.
    WB: 1/100 - 1/1000. Predicted molecular weight: 183 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      Vitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
    • 细胞定位
      Secreted
    • 别名
      • Vg antibody
      • VTG antibody
      • VTG1 antibody

    图片



    • Predicted band size : 183 kDa

      Image from Fan X et al, J Biol Chem. 2010 Oct 22;285(43):32946-53. Epub 2010 Aug 20, Fig 6. DOI 10.1074/jbc.M110.158295

    实验方案

    文献

    This product has been referenced in:
    • Li Q  et al. FOXL2 down-regulates vitellogenin expression at mature stage in Eriocheir sinensis. Biosci Rep 35:N/A (2015). Read more (PubMed: 26430246) »
    • Fan X  et al. Selective yolk deposition and mannose phosphorylation of lysosomal glycosidases in zebrafish. J Biol Chem 285:32946-53 (2010). WB ; Zebrafish . Read more (PubMed: 20729204) »

    See all 2 Publications for this product

    客户评价及客户问答

    There are currently no Customer reviews or Questions for ab36804.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册