Anti-Vitamin D Receptor抗体(ab82566)

概述

  • 产品名称Anti-Vitamin D Receptor抗体
    参阅全部 Vitamin D Receptor 一抗
  • 描述
    兔多克隆抗体to Vitamin D Receptor
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Chicken, Cow, Pig, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Vitamin D Receptor .

    (Peptide available as ab90849.)

  • 阳性对照
    • This antibody gave a positive signal in the following human tissue lysates: Skin; Thyroid; Small intestine; Bone marrow; Kidney.

性能

应用

Our Abpromise guarantee covers the use of ab82566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 48 kDa).

靶标

  • 功能Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
  • 疾病相关Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1 25 dihydroxyvitamin D3 receptor antibody
    • 1 antibody
    • 1,25 dihydroxyvitamin D3 receptor antibody
    • 1,25-@dihydroxyvitamin D3 receptor antibody
    • 25-dihydroxyvitamin D3 receptor antibody
    • Member 1 antibody
    • NR1I1 antibody
    • Nuclear receptor subfamily 1 group I member 1 antibody
    • PPP1R163 antibody
    • Protein phosphatase 1, regulatory subunit 163 antibody
    • VDR antibody
    • VDR_HUMAN antibody
    • Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody
    • Vitamin D hormone receptor antibody
    • Vitamin D nuclear receptor variant 1 antibody
    • Vitamin D receptor antibody
    • Vitamin D3 receptor antibody
    see all

Anti-Vitamin D Receptor antibody 图像

  • All lanes : Anti-Vitamin D Receptor antibody (ab82566) at 1 µg/ml

    Lane 1 : Human skin tissue lysate - total protein (ab30166)
    Lane 2 : Human thyroid normal tissue lysate - total protein (ab29636)
    Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 4 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 5 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 94 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Anti-Vitamin D Receptor antibody (ab82566) at 1 µg/ml + Recombinant Human Vitamin D Receptor protein (ab82068) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 seconds

Anti-Vitamin D Receptor antibody (ab82566)参考文献

ab82566 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. This antibody can be used with serum samples as long as your serum samples contain vitamin D at detectable level. You would need to optimise the protocol to obtain a lysate suitable for loading in the WB gel. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"