Anti-Vitamin D Binding protein抗体[EP2923Y] (ab81307)


  • 产品名称Anti-Vitamin D Binding protein抗体[EP2923Y]
    参阅全部 Vitamin D Binding protein 一抗
  • 描述
    兔单克隆抗体[EP2923Y] to Vitamin D Binding protein
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues within Human Vitamin D Binding Protein.

  • 阳性对照
    • Human liver tissue. Human plasma.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Vitamin D Binding protein antibody (HRP) [EP2923Y] (ab197711)

    Anti-Vitamin D Binding protein antibody (Alexa Fluor® 647) [EP2923Y] (ab198339)




Our Abpromise guarantee covers the use of ab81307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/20000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
    • 序列相似性Belongs to the ALB/AFP/VDB family.
      Contains 3 albumin domains.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DBP antibody
      • DBP/GC antibody
      • GC antibody
      • Gc globulin antibody
      • Gc-globulin antibody
      • GRD3 antibody
      • Group specific component antibody
      • Group specific component vitamin D binding protein antibody
      • Group-specific component antibody
      • hDBP antibody
      • VDB antibody
      • VDBG antibody
      • VDBP antibody
      • Vitamin D binding alpha globulin antibody
      • Vitamin D-binding protein antibody
      • VTDB_HUMAN antibody
      see all

    Anti-Vitamin D Binding protein antibody [EP2923Y] 图像

    • Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307) at 1/20000 dilution + Human plasma lysate at 10 µg

      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

      Predicted band size : 53 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)

      Exposure time : 3 minutes

      Blocking and Diluting buffer 5% NFDM /TBST 

    • Immunohistochemical analysis of paraffin-embedded human liver using ab81307 at a 1/100 dilution.

    Anti-Vitamin D Binding protein antibody [EP2923Y] (ab81307)参考文献

    ab81307 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Brain lysate, Liver lysate and human serum)
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Loading amount 30 µg
    Specification Brain lysate, Liver lysate and human serum
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Dr. Ruma Raha-Chowdhury

    Verified customer

    提交于 Jan 28 2016