概述

  • 产品名称Anti-Visfatin抗体
    参阅全部 Visfatin 一抗
  • 描述
    兔多克隆抗体to Visfatin
  • 特异性This antibody reacts with PBEF
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide: HRTPAGNFVT LEEGKGDLEE YGQDLLHTVF KNGKVTKSYS FDEIRKNAQL , corresponding to amino acids 434-482 of Human PBEF

  • 阳性对照
    • Jurkat cell lysate and human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab58640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
  • 组织特异性Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
  • 通路Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.
  • 序列相似性Belongs to the NAPRTase family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1110035O14Rik antibody
    • AI314458 antibody
    • AI480535 antibody
    • DKFZP666B131 antibody
    • EC 2.4.2.12 antibody
    • MGC117256 antibody
    • NAmPRTase antibody
    • Nampt antibody
    • NAMPT_HUMAN antibody
    • Nicotinamide phosphoribosyltransferase antibody
    • PBEF 1 antibody
    • PBEF antibody
    • PBEF1 antibody
    • Pre B cell colony enhancing factor 1 antibody
    • Pre B cell colony enhancing factor antibody
    • Pre B cell enhancing factor antibody
    • Pre-B cell-enhancing factor antibody
    • Pre-B-cell colony-enhancing factor 1 antibody
    • VF antibody
    • Visfatin antibody
    see all

Anti-Visfatin antibody 图像

  • Anti-Visfatin antibody (ab58640) at 1.25 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk in PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
    Gel concentration: 12%
  • ab58640 at a 4.0µg/ml staining PBEF in the epithelial cells of human renal tubules (indicated with arrows). Magnification: 400X
  • ICC/IF image of ab58640 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58640, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Visfatin antibody (ab58640)参考文献

This product has been referenced in:
  • Zhang J  et al. Extended wakefulness: compromised metabolics in and degeneration of locus ceruleus neurons. J Neurosci 34:4418-31 (2014). Mouse . Read more (PubMed: 24647961) »
  • Yamamoto T  et al. Nicotinamide mononucleotide, an intermediate of NAD+ synthesis, protects the heart from ischemia and reperfusion. PLoS One 9:e98972 (2014). Mouse . Read more (PubMed: 24905194) »

See all 2 Publications for this product

Product Wall

Thank you for your reply.

I have updated the address on the order, and it should arrive tomorrow. I look forward to receiving your protocol and hopefully resolving this issue.

Thanks again for your information. Please let me know if...

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Thank you for your call yesterday and for your email.

I'm sending a free of charge vial of ab58640 on the order ***, which should arrive tomorrow. This vial is from the same production batch as the first vial that you used in 2009 so I'm hope...

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