概述

  • 产品名称Anti-VIP抗体
    参阅全部 VIP 一抗
  • 描述
    兔多克隆抗体to VIP
  • 特异性The antiserum supplied has been evaluated for its ability to stain ganglion cells in both the superficial and deep plexus of the wall of the human small bowel.
  • 经测试应用适用于: IHC-P, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig, Cat, Dog, Human
  • 免疫原

    Synthetic porcine vasoactive intestinal peptide,

    SAKKYLESLIX

    (amino acids 18-28), bound to KLH with carbodiimide.

  • 阳性对照
    • Small bowel tissue with plexus attached. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HCT116.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab78536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/1000.
IHC-Fr 1/500 - 1/1000.
ICC/IF 1/200.

靶标

Anti-VIP antibody 图像

  • IHC image of ab78536 staining in mouse intestine formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78536, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab78536 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab78536 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-VIP antibody (ab78536)参考文献

This product has been referenced in:
  • De Vadder F  et al. Vasoactive intestinal peptide is a local mediator in a gut-brain neural axis activating intestinal gluconeogenesis. Neurogastroenterol Motil 27:443-8 (2015). IHC-P ; Rat . Read more (PubMed: 25586379) »
  • Raghavan S  et al. Successful implantation of bioengineered, intrinsically innervated, human internal anal sphincter. Gastroenterology 141:310-9 (2011). IHC-P ; Human . Read more (PubMed: 21463628) »

See all 2 Publications for this product

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Thank you for contacting Abcam regarding ab78536.

I am sorry that you have been experiencing difficulties with this antibody in your experiments. I have reviewed the protocol information you provided and I agree that this antibody does not a...

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Vielen Dank für Ihre Anfrage. Leider wurde die genaue Konzentration dieses Antikörpers nicht gemessen. Es handelt sich hierbei um ein un-aufgereinigtes Antiserum und es kann höchstens die Gesamtproteinkonzentration gemessen werden. Als genereller Anhal...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"