Anti-VIP抗体(ab6184)

概述

  • 产品名称
  • 描述
    羊多克隆抗体to VIP
  • 特异性
    The reactivity against other peptides has not been examined.
  • 经测试应用
    适用于: IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Guinea pig, Cow, Dog, Human, Pig
  • 免疫原

    Synthetic peptide:

    H-HSDAVFTDNYTRLRKQMAVKKYLNSILNQ

    conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 45-72 of Pig VIP.

  • 常规说明
    Excellent antibody for immunohistochemistry for both the peripheral and central nervous system.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 纯度
    Whole antiserum
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/1000.
IHC-P 1/1000.

靶标

文献

ab6184 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (brain)
Specification
brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

提交于 Oct 11 2006

Thank you for your enquiry. To our knowledge this antibody has yet to be used in this application. All the information we have available is that which is listed on the product datasheets. We have no further information about this application. Best of l...

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I'm sorry to hear you are having problems with ab6184. I would like to recommend the following: -an enzymatic antigen retrieval step -fixation using Zamboni's fixative (4%PFA + 15% picric acid). Please let me know if these modifications to your p...

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Thank you fo your patience. We now have an answer to your initial enquiry.We can confirm that the antisera is suitable for use in all species identified on the datasheet, ie, Human, Mouse, Rat, Cow, Dog, Guinea Pig, Pig and Rabbit. The statement "Speci...

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We have not tested this antibody in chick.

As far as we are aware, the antibody has only been tested using Zamboni's, so we do not know if it will work with paraffin embedded tissues. The work with rabbit was carried out by the antibody's originators, a research lab in Australia, however it h...

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The fixative used for this antibody is Zamboni's (buffered 4% paraformaldehyde with 15% picric acid). Sympathetic and enteric nerve fibres stain particularly well, cell bodies are visible but less intensely stained.

We can assure you that this antibody is in routine use for guinea-pig enteric and sympathetic nervous system and with Zamboni's fixative. Several secondary antibodies are used and found appropriate.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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