概述

  • 产品名称Anti-VIP抗体
    参阅全部 VIP 一抗
  • 描述
    兔多克隆抗体to VIP
  • 特异性ab22736 reacts with Vasoactive Intestinal (poly)Peptide (VIP).
  • 经测试应用适用于: IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Sheep, Dog, Human, Pig
  • 免疫原

    Full length native protein (purified) (Pig) conjugated to KLH by a Glutaraldehyde linker.

  • 阳性对照
    • Human, rat and porcine colon and spinal cord.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr
IHC-P
  • 应用说明IHC-Fr: 1/400 - 1/1600.
    IHC-P: 1/400 - 1/1600.
    Notes: Dilute to working strength with PBS containing 0.1% w/v sodium azide.
    Store diluted antibodies at 2-4°C(do not freeze) and use within 1 month.
    using overnight incubation and indirect immunofluorescence procedure.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-VIP antibody 图像

    • ab22736 staining VIP in jejunum tissue section of pig by Immunohistochemistry (frozen sections). Tissue underwent fixation in paraformaldehyde and incubated with primary antibody, diluted 1/200 for 24 hours. A Texas Red® conjugated goat polyclonal to rabbit, dilution 1/400 was used as the secondary.

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    • ab22736 staining VIP in sheep abomasum tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in paraformaldehyde without undergoing permeabilization and blocking steps. The primary antibody was diluted 1/1200 and incubated with sample for 24 hours. A FITC-conjugated goat polyclonal (IgG whole molecule) to rabbit IgG was diluted 1/400 and used as secondary. 

    Anti-VIP antibody (ab22736)参考文献

    ab22736 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Pig Tissue sections (jejunum)
    Specification jejunum
    Fixative Paraformaldehyde
    Permeabilization No
    Username

    Prof. Marcin Arciszewski

    Verified customer

    提交于 Nov 06 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Sheep Tissue sections (abomasum)
    Specification abomasum
    Fixative Paraformaldehyde
    Permeabilization No
    Username

    Prof. Marcin Arciszewski

    Verified customer

    提交于 Oct 22 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Dog Tissue sections (duodenum)
    Specification duodenum
    Fixative Paraformaldehyde
    Permeabilization No
    Username

    Prof. Marcin Arciszewski

    Verified customer

    提交于 Jun 22 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"