Anti-Vinexin抗体(ab68222)

概述

  • 产品名称
    Anti-Vinexin抗体
    参阅全部 Vinexin 一抗
  • 描述
    小鼠多克隆抗体to Vinexin
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Vinexin protein (Human)

  • 阳性对照
    • Vinexin transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab68222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 75 kDa).

靶标

  • 相关性
    Vinexin is a focal adhesion protein with a sorbin homology (SoHo) domain as well as 3 SH3 domains. It exists as at least three splice variants - alpha, beta and gamma. It is thought to play a key role in cell adhesion, cell spreading and migration and cytoskeletal organisation. It has also been implicated signaling due to its interaction with the extracellular signal-regulated kinase ERK. Binds to vinculin at sites of cell-cell contact.
  • 细胞定位
    Localized at cell-extracellular matrix junctions. Both isoforms were localized at focal adhesion and cell-cell adhesion sites, vinexin beta was also found in the nucleus.
  • 数据库链接
  • 别名
    • SCAM 1 antibody
    • SCAM1 antibody
    • SH3 containing adapter molecule 1 antibody
    • SH3 domain containing adapter molecule 1 antibody
    • SH3D4 antibody
    • Sorbin and SH3 domain containing 3 antibody
    • Sorbin and SH3 domain containing protein 3 antibody
    • Sorbs3 antibody
    • Vinexin alpha antibody
    • Vinexin antibody
    • Vinexin beta (SH3 containing adaptor molecule 1) antibody
    see all

图片

  • All lanes : Anti-Vinexin antibody (ab68222) at 1/500 dilution

    Lane 1 : Vinexin transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 75 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab68222 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Embryonic Fibroblast (MEF))
Specification
Mouse Embryonic Fibroblast (MEF)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton
Username

Abcam user community

Verified customer

提交于 Nov 16 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cell line ( cervical cancer))
Specification
HeLa cell line ( cervical cancer)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton
Username

Abcam user community

Verified customer

提交于 Nov 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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