Anti-Vinculin抗体- N-terminal (ab82418)


  • 产品名称Anti-Vinculin抗体- N-terminal
    参阅全部 Vinculin 一抗
  • 描述
    兔多克隆抗体to Vinculin - N-terminal
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the N-terminal region of human Vinculin.

  • 阳性对照
    • K562 cell line lysate. Mouse lung tissue lysate.



Our Abpromise guarantee covers the use of ab82418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
WB 1/50 - 1/100. Predicted molecular weight: 124 kDa.
ELISA 1/1000.


  • 功能Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
  • 组织特异性Metavinculin is muscle-specific.
  • 疾病相关Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) [MIM:611407]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in VCL are the cause of cardiomyopathy familial hypertrophic type 15 (CMH15) [MIM:613255]. It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
  • 序列相似性Belongs to the vinculin/alpha-catenin family.
  • 结构域Exists in at least two conformations. When in the closed, 'inactive' conformation, extensive interactions between the head and tail domains prevent detectable binding to most of its ligands. It takes on an 'active' conformation after cooperative and simultaneous binding of two different ligands. This activation involves displacement of the head-tail interactions and leads to a significant accumulation of ternary complexes. The active form then binds a number of proteins that have both signaling and structural roles that are essential for cell adhesion.
    The N-terminal globular head (Vh) comprises of subdomains D1-D4. The C-terminal tail (Vt) binds F-actin and cross-links actin filaments into bundles. An intramolecular interaction between Vh and Vt masks the F-actin-binding domain located in Vt. The binding of talin and alpha-actinin to the D1 subdomain of vinculin induces a helical bundle conversion of this subdomain, leading to the disruption of the intramolecular interaction and the exposure of the cryptic F-actin-binding domain of Vt. Vt inhibits actin filament barbed end elongation without affecting the critical concentration of actin assembly.
  • 翻译后修饰Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques.
    Aceylated; mainly by myristic acid but also small amount of palmitic acid.
  • 细胞定位Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CMD1W antibody
    • CMH15 antibody
    • Epididymis luminal protein 114 antibody
    • HEL114 antibody
    • Metavinculin antibody
    • MV antibody
    • MVCL antibody
    • OTTHUMP00000019861 antibody
    • OTTHUMP00000019862 antibody
    • VCL antibody
    • VINC antibody
    • VINC_HUMAN antibody
    • Vinculin antibody
    see all

Anti-Vinculin antibody - N-terminal 图像

  • All lanes : Anti-Vinculin antibody - N-terminal (ab82418) at 1/50 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Mouse lung tissue lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size : 124 kDa
    Observed band size : 124 kDa
  • ab82418 at 1/100 dilution staining Vinculin -N- terminal in human skeletal tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

Anti-Vinculin antibody - N-terminal (ab82418)参考文献

ab82418 has not yet been referenced specifically in any publications.

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