Anti-Vimentin (phospho S72)抗体[EP1070Y] (ab52944)


  • 产品名称
    Anti-Vimentin (phospho S72)抗体[EP1070Y]
    参阅全部 Vimentin 一抗
  • 描述
    兔单克隆抗体[EP1070Y] to Vimentin (phospho S72)
  • 特异性
    ab52944 only detects Vimentin phosphorylated at Serine 72.
  • 经测试应用
    适用于: WB, IPmore details
    不适用于: Flow Cyt,IHC-Fr or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic acetylated peptide corresponding to residues surrounding Ser72 of Vimentin

  • 阳性对照
    • HeLa cell lysate (untreated and Calyculin A treated).
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.




Our Abpromise guarantee covers the use of ab52944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100000 - 1/1e+006. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IP 1/40.
  • 应用说明
    Is unsuitable for Flow Cyt,IHC-Fr or IHC-P.
  • 靶标

    • 功能
      Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
      Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
    • 组织特异性
      Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
    • 疾病相关
      Cataract 30
    • 序列相似性
      Belongs to the intermediate filament family.
    • 结构域
      The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
      The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    • 翻译后修饰
      Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
      O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
      S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
    • 细胞定位
    • Information by UniProt
    • 数据库链接
    • 形式
      Vimentin is found in connective tissue and in the cytoskeleton.
    • 别名
      • CTRCT30 antibody
      • Epididymis luminal protein 113 antibody
      • FLJ36605 antibody
      • HEL113 antibody
      • VIM antibody
      • VIME_HUMAN antibody
      • Vimentin antibody
      see all

    Anti-Vimentin (phospho S72) antibody [EP1070Y] 图像

    • Primary antibody dilution: 1/1000

      Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

      Secondary antibody dilution: 1/2500

      Blocking & dilution buffer: 5% NFDM/TBST

      Lane 1 sample: Vimentin (pS72) phospho peptide

      Lane 2 sample: Vimentin non-phospho peptide

      Exposure time: 3 minutes

    • All lanes : Anti-Vimentin (phospho S72) antibody [EP1070Y] (ab52944) at 1/1000000 dilution

      Lane 1 : HeLa cell lysate untreated
      Lane 2 : HeLa cell lysate treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 54 kDa
      Observed band size : 54 kDa

    Anti-Vimentin (phospho S72) antibody [EP1070Y] (ab52944)参考文献

    This product has been referenced in:
    • Goto H  et al. Phospho-Specific Antibody Probes of Intermediate Filament Proteins. Methods Enzymol 568:85-111 (2016). Read more (PubMed: 26795468) »
    • Mähönen AJ  et al. Culture medium induced vimentin reorganization associates with enhanced baculovirus-mediated gene delivery. J Biotechnol 145:111-9 (2010). Read more (PubMed: 19903502) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your patience in this matter. The originator of ab52944 (Vimentin (phospho S72) antibody [EP1070Y]) has confirmed that the recommended dilution for WB is 1:1000000. Please don't hesitate to contact us again if you have any further q...

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