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RabMAb

Anti-Vimentin (phospho S38)抗体[EP1069Y] (ab52942)

概述

  • 产品名称
    Anti-Vimentin (phospho S38)抗体[EP1069Y]
    参阅全部 Vimentin 一抗
  • 描述
    兔单克隆抗体[EP1069Y] to Vimentin (phospho S38)
  • 特异性
    ab52942 only detects Vimentin phosphorylated at Serine 38.
  • 经测试应用
    适用于: WB, ICC/IFmore details
    不适用于: Flow Cyt,IHC-Fr,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Vimentin aa 1-100 (phospho S38). The exact sequence is proprietary.
    Database link: P08670

  • 阳性对照
    • WB: HeLa cell lysate treated with Calyculin A.
  • 常规说明

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab52942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 24073199
  • 应用说明
    Is unsuitable for Flow Cyt,IHC-Fr,IHC-P or IP.
  • 靶标

    • 功能
      Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
      Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
    • 组织特异性
      Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
    • 疾病相关
      Cataract 30
    • 序列相似性
      Belongs to the intermediate filament family.
    • 结构域
      The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
      The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    • 翻译后修饰
      Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
      O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
      S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 形式
      Vimentin is found in connective tissue and in the cytoskeleton.
    • 别名
      • CTRCT30 antibody
      • Epididymis luminal protein 113 antibody
      • FLJ36605 antibody
      • HEL113 antibody
      • VIM antibody
      • VIME_HUMAN antibody
      • Vimentin antibody
      see all

    Anti-Vimentin (phospho S38) antibody [EP1069Y] 图像

    • All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/1000000 dilution

      Lane 1 : HeLa whole cell lysate - untreated
      Lane 2 : HeLa whole cell lysate - treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 54 kDa
      Observed band size : 54 kDa


      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot blot analysis of Vimentin (pS38) peptide (Lane 1) and Vimentin non-phospho peptide (Lane 2) labelling Vimentin (phospho S38) with ab52942 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • All lanes : Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942) at 1/200000 dilution

      Lane 1 : HeLa cell lysate untreated
      Lane 2 : HeLa cell lysate Calyculin A treated

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 54 kDa
      Observed band size : 54 kDa

    Anti-Vimentin (phospho S38) antibody [EP1069Y] (ab52942)参考文献

    This product has been referenced in:

    See all 5 Publications for this product

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