Anti-Vimentin抗体[Vim 3B4] (ab28028)


  • 产品名称Anti-Vimentin抗体[Vim 3B4]
    参阅全部 Vimentin 一抗
  • 描述
    小鼠单克隆抗体[Vim 3B4] to Vimentin
  • 特异性Ab28028 recognises Vimentin
  • 经测试应用适用于: WB, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Human, Xenopus laevis
  • 免疫原

    Full length native protein (purified): Vimentin isolated from bovine eye lens.

  • 表位Ab28028 reacts with an epitope located in the coil 2 part of the vimentin rod domain.
  • 阳性对照



Our Abpromise guarantee covers the use of ab28028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/200. Predicted molecular weight: 54 kDa.
IHC-Fr 1/100 - 1/200. The antibody can be used for labeling acetone fixed, frozen sections.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • 功能Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 组织特异性Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • 疾病相关Cataract 30
  • 序列相似性Belongs to the intermediate filament family.
  • 结构域The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • 翻译后修饰Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 形式Vimentin is found in connective tissue and in the cytoskeleton.
  • 别名
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Anti-Vimentin antibody [Vim 3B4] 图像

  • ab28028 staining of tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  • ab28028 staining of tonsil by Immunohistochemistry(Formalin-fixed paraffin-embedded sections)

  •  ab28028 at 1/200 dilution staining vimentin in human lung cancer cells. The Vimentin staining is shown in red. The cells were counterstained with DAPI. Please see accompanying abreview for additional information.

    See Abreview

  • ab28028 staining Vimentin in human fibrosarcoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 1% Triton x100. Samples were incubated with primary antibody (1/100) for 1 hour at 20°C. An Alexa Fluor®488-conjugated donkey monoclonal to mouse IgG (ab150105) was used at dilution at 1/500 as secondary antibody.

    See Abreview

Anti-Vimentin antibody [Vim 3B4] (ab28028)参考文献

This product has been referenced in:
  • Buchholz M  et al. A multistep high-content screening approach to identify novel functionally relevant target genes in pancreatic cancer. PLoS One 10:e0122946 (2015). ICC . Read more (PubMed: 25849100) »
  • Beaudet MJ  et al. High yield extraction of pure spinal motor neurons, astrocytes and microglia from single embryo and adult mouse spinal cord. Sci Rep 5:16763 (2015). Read more (PubMed: 26577180) »

See all 11 Publications for this product

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L'anticorps ab28028 (Anti-Vimentin [Vim 3B4]) est un supernageant, la concentration ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibrosarcoma)
Specification fibrosarcoma
Fixative Paraformaldehyde
Permeabilization Yes - 1% TX-100

Abcam user community

Verified customer

提交于 Oct 16 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Lung cancer)
Specification Lung cancer
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Dr. Da Zhu

Verified customer

提交于 Mar 26 2009