Anti-Vimentin抗体[VI-RE/1] (Phycoerythrin) (ab49918)

概述

  • 产品名称Anti-Vimentin抗体[VI-RE/1] (Phycoerythrin)
    参阅全部 Vimentin 一抗
  • 描述
    小鼠单克隆抗体[VI-RE/1] to Vimentin (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特异性The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament protein expressed on a wide variety of mesenchymal and mesodermal cell types.
  • 经测试应用适用于: ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Pig
  • 免疫原

    Recombinant human vimentin.

  • 阳性对照
    • ICC/IF: HeLa cells
  • 常规说明

    Conjugated with R-Phycoerythrin (PE) under optimum conditions.

性能

应用

Our Abpromise guarantee covers the use of ab49918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use a concentration of 10 µg/ml.

Working concentrations should be determined by the investigator.





ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 组织特异性Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • 疾病相关Cataract 30
  • 序列相似性Belongs to the intermediate filament family.
  • 结构域The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • 翻译后修饰Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 形式Vimentin is found in connective tissue and in the cytoskeleton.
  • 别名
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin) 图像

  • ab49918 staining Vimentinn in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab49918 at a working concentration of 10μg/ml (shown in red) and ab185031, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow cytometric analysis of Human stromal endometrial somatic stem cells, staining Vimentin with ab49918.

    Gating Strategy: Live cells

Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin) (ab49918)参考文献

This product has been referenced in:
  • Cervelló I  et al. Reconstruction of endometrium from human endometrial side population cell lines. PLoS One 6:e21221 (2011). Flow Cyt ; Human . Read more (PubMed: 21712999) »
  • Basu D  et al. Evidence for mesenchymal-like sub-populations within squamous cell carcinomas possessing chemoresistance and phenotypic plasticity. Oncogene : (2010). Flow Cyt . Read more (PubMed: 20498638) »

See all 2 Publications for this product

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