Customer reviews and FAQs

概述

  • 产品名称Anti-Vimentin抗体[RV203]
    参阅全部 Vimentin 一抗
  • 描述
    小鼠单克隆抗体[RV203] to Vimentin
  • 特异性This antibody reacts exclusively with vimentin
  • 经测试应用适用于: IHC-P, Flow Cyt, ICC, IHC-Fr, WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Goat, Hamster, Dog, Human, Pig
    不与反应: Mouse
  • 免疫原

    Cytoskeletal vimentin extract of calf lens.

  • 阳性对照

性能

应用

Our Abpromise guarantee covers the use of ab8979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt 1/100 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Recommended range is 1/100 - 1/200 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
WB 1/100 - 1/1000. Detects a band of approximately 57 kDa.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 组织特异性Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • 疾病相关Cataract 30
  • 序列相似性Belongs to the intermediate filament family.
  • 结构域The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • 翻译后修饰Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
    see all
  • 形式Vimentin is found in connective tissue and in the cytoskeleton.
  • 别名
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Anti-Vimentin antibody [RV203] 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)
    IHC image of ab8979 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8979, µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (ab8979)
    Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (ab8979)
    Immunohistochemistry on frozen section of human colon showing positive staining in connective tissue cells and no reactivity in epithelial cells
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)
    Immunohistochemistry on paraffin section of human colon
  • Flow Cytometry - Vimentin antibody [RV203] (ab8979)
    Flow Cytometry - Vimentin antibody [RV203] (ab8979)
    Overlay histogram showing HeLa cells stained with ab8979 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8979, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

Anti-Vimentin antibody [RV203] (ab8979)参考文献

This product has been referenced in:
  • Weng M  et al. The E3 ubiquitin ligase NEDD4 is translationally upregulated and facilitates pancreatic cancer. Oncotarget 8:20288-20296 (2017). WB ; Human . Read more (PubMed: 28423617) »
  • Sohn J  et al. The subventricular zone continues to generate corpus callosum and rostral migratory stream astroglia in normal adult mice. J Neurosci 35:3756-63 (2015). IHC-FoFr ; Mouse . Read more (PubMed: 25740506) »

See all 3 Publications for this product

发表研究结果有使用 ab8979？请让我们知道，以便我们可以引用本数据表中的参考文章。

Product Wall

提交 ab8979 的 abreview 提交 ab8979 的问题

1 Abreview or Q&A

10
Votes: Highest First

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [RV203]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Reacts exclusively with vimentin)
Specification Reacts exclusively with vimentin
Fixative Methanol
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% BSA
Read More
Username

Abcam user community

Verified customer

提交于 Jan 24 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"