概述

  • 产品名称
    Anti-Vimentin抗体
    参阅全部 Vimentin 一抗
  • 描述
    兔多克隆抗体to Vimentin
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant human protein purified from E.coli (His-Vimentin)

  • 阳性对照
    • RD2, C6, HeLa and NIH3T3 cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab71144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IP Use at an assay dependent concentration. Use 4 µl for 200 µg lysate.

靶标

  • 功能
    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 组织特异性
    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • 疾病相关
    Cataract 30
  • 序列相似性
    Belongs to the intermediate filament family.
  • 结构域
    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • 翻译后修饰
    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 形式
    Vimentin is found in connective tissue and in the cytoskeleton.
  • 别名
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

图片

  • All lanes : Anti-Vimentin antibody (ab71144) at 1/5000 dilution

    Lane 1 : RD2 cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : NIH3T3 cell lysate


    Predicted band size : 54 kDa
    Observed band size : 54 kDa

文献

This product has been referenced in:
  • Huang C  et al. Upregulation of H19 promotes invasion and induces epithelial-to-mesenchymal transition in esophageal cancer. Oncol Lett 10:291-296 (2015). WB ; Human . Read more (PubMed: 26171017) »
  • Maejima Y  et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med 19:1478-88 (2013). IHC . Read more (PubMed: 24141421) »

See all 4 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela)
Loading amount
100 µg
Specification
Hela
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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提交于 Jun 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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