概述

  • 产品名称
    Anti-Vimentin抗体
    参阅全部 Vimentin 一抗
  • 描述
    山羊多克隆抗体to Vimentin
  • 特异性
    No reactivity was observed with the other families of intermediate filaments, including, desmin, keratin, neurofilaments and glial filaments.The antibody localizes vimentin in normal and pathological tissue of mesenchymal derivation. The antibody shows wide cross-reactivity among mammalian species.
  • 经测试应用
    适用于: ICC/IF, IHC-P, IHC-Fr, WB, ICCmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: a wide range of other species
  • 免疫原

    Other Immunogen Type corresponding to Vimentin. vimentin from cultured human foreskin fibroblasts.

  • 阳性对照
  • 常规说明
    If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. This goat antiserum was maintained at pH 5.0 for 40 minutes to meet U.S.D.A. requirements.

性能

应用

Our Abpromise guarantee covers the use of ab11256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-P 1/20. Localizes vimentin in fibroblasts, endothelial cells, lymphoid tissues and melanocytes, as well as tumors derived from these cells including sarcomas, lymphomas, melanomas, and their metastatic lesions.
IHC-Fr 1/20.
WB 1/50. Detects a band of approximately 58 kDa.

The antibody specifically stains the vimentin band using total cell or tissue extracts.

ICC Use at an assay dependent concentration. On normal and pathological tissue of mesenchymal derivation.

靶标

  • 功能
    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 组织特异性
    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • 疾病相关
    Cataract 30
  • 序列相似性
    Belongs to the intermediate filament family.
  • 结构域
    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • 翻译后修饰
    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 形式
    Vimentin is found in connective tissue and in the cytoskeleton.
  • 别名
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

图片

  • ii Panels i-iii, TGF-ß1 induces EMT-type changes in primary hepatocytes. Immunofluorescence staining for E-cadherin (green) and vimentin (red) in primary hepatocytes were evaluated by confocal laser microscopy. DAPI stained nuclei blue. There was apparent near loss of cell membrane-bound E-cadherin in TGF-ß1-treated cells in a time-dependent manner. Diffuse vimentin staining was observed in control cells, and TGF-ß1-treated hepatocytes exhibited a fibrillar vimentin pattern most noted at 48 and 72 h. Also note that TGF-ß1 caused hepatocytes to assume a spindle-shaped morphology, which was not observed in controls. Scale bar, 50 µm.
  • Immunocytochemistry/ Immunofluorescence analysis of mouse sertoli cells labeling Vimentin with ab11256 at 1/20 dilution. Cells were fixed with methanol and blocked with 5% serum for 20 minutes at 25 °C. A polyclonal Donkey anti-goat Dylight® 488 was used as the secondary antibody at 1/400 dilution.

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文献

This product has been referenced in:
  • Nagy BM  et al. Lack of ABCG2 Leads to Biventricular Dysfunction and Remodeling in Response to Hypoxia. Front Physiol 8:98 (2017). ICC/IF ; Mouse . Read more (PubMed: 28270772) »
  • Chorghade S  et al. Poly(A) tail length regulates PABPC1 expression to tune translation in the heart. Elife 6:N/A (2017). WB, IF ; Mouse . Read more (PubMed: 28653618) »

See all 15 Publications for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Sample
Human Cell (breast cancer cell lines)
Specification
breast cancer cell lines
Permeabilization
Yes - 0.3% Triton X-100 in TBS
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 18 2014

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (Breast cancer cell lines)
Specification
Breast cancer cell lines
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Dec 10 2014

Yes this antibody has been tested on human paraffin embedded tissue followed by HIER.

The following publication used our antibody in this application on human placenta:

Lockwood CJ et al. Progestin and thrombin regulate tissue factor ...

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The antigen retrieval was performed using 0.1% Trypsin Enzyme incubation for 15 - 25 min. The enzyme was incubated at a temperature of 37°C

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (sertoli cells)
Specification
sertoli cells
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Elad Fadlon

Verified customer

提交于 Aug 06 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Heart)
Specification
Heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Feb 21 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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