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Other Immunogen Type corresponding to Vimentin. vimentin from cultured human foreskin fibroblasts.
Our Abpromise guarantee covers the use of ab11256 in the following tested applications.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/20. Localizes vimentin in fibroblasts, endothelial cells, lymphoid tissues and melanocytes, as well as tumors derived from these cells including sarcomas, lymphomas, melanomas, and their metastatic lesions.|
|WB||1/50. Detects a band of approximately 58 kDa.
The antibody specifically stains the vimentin band using total cell or tissue extracts.
|ICC||Use at an assay dependent concentration. On normal and pathological tissue of mesenchymal derivation.|
Immunocytochemistry/ Immunofluorescence analysis of mouse sertoli cells labeling Vimentin with ab11256 at 1/20 dilution. Cells were fixed with methanol and blocked with 5% serum for 20 minutes at 25 °C. A polyclonal Donkey anti-goat Dylight® 488 was used as the secondary antibody at 1/400 dilution.