概述

  • 产品名称Anti-Vimentin抗体
    参阅全部 Vimentin 一抗
  • 描述
    山羊多克隆抗体to Vimentin
  • 特异性No reactivity was observed with the other families of intermediate filaments, including, desmin, keratin, neurofilaments and glial filaments.The antibody localizes vimentin in normal and pathological tissue of mesenchymal derivation. The antibody shows wide cross-reactivity among mammalian species.
  • 经测试应用适用于: ICC/IF, IHC-P, IHC-Fr, WB, ICCmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: a wide range of other species
  • 免疫原

    Other Immunogen Type corresponding to Vimentin. vimentin from cultured human foreskin fibroblasts.

  • 阳性对照
  • 常规说明If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. This goat antiserum was maintained at pH 5.0 for 40 minutes to meet U.S.D.A. requirements.

性能

应用

Our Abpromise guarantee covers the use of ab11256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-P 1/20. Localizes vimentin in fibroblasts, endothelial cells, lymphoid tissues and melanocytes, as well as tumors derived from these cells including sarcomas, lymphomas, melanomas, and their metastatic lesions.
IHC-Fr 1/20.
WB 1/50. Detects a band of approximately 58 kDa.

The antibody specifically stains the vimentin band using total cell or tissue extracts.

ICC Use at an assay dependent concentration. On normal and pathological tissue of mesenchymal derivation.

靶标

  • 功能Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 组织特异性Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • 疾病相关Cataract 30
  • 序列相似性Belongs to the intermediate filament family.
  • 结构域The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • 翻译后修饰Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 形式Vimentin is found in connective tissue and in the cytoskeleton.
  • 别名
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Anti-Vimentin antibody 图像

  • ii Panels i-iii, TGF-ß1 induces EMT-type changes in primary hepatocytes. Immunofluorescence staining for E-cadherin (green) and vimentin (red) in primary hepatocytes were evaluated by confocal laser microscopy. DAPI stained nuclei blue. There was apparent near loss of cell membrane-bound E-cadherin in TGF-ß1-treated cells in a time-dependent manner. Diffuse vimentin staining was observed in control cells, and TGF-ß1-treated hepatocytes exhibited a fibrillar vimentin pattern most noted at 48 and 72 h. Also note that TGF-ß1 caused hepatocytes to assume a spindle-shaped morphology, which was not observed in controls. Scale bar, 50 µm.

Anti-Vimentin antibody (ab11256)参考文献

This product has been referenced in:
  • Chen YY  et al. Capture and Identification of Heterogeneous Circulating Tumor Cells Using Transparent Nanomaterials and Quantum Dots-Based Multiplexed Imaging. J Cancer 7:69-79 (2016). ICC/IF ; Human . Read more (PubMed: 26722362) »
  • Szwarc MM  et al. Aberrant Activation of the RANK Signaling Receptor Induces Murine Salivary Gland Tumors. PLoS One 10:e0128467 (2015). Mouse . Read more (PubMed: 26061636) »

See all 7 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Sample Human Cell (breast cancer cell lines)
Specification breast cancer cell lines
Permeabilization Yes - 0.3% Triton X-100 in TBS
Fixative Paraformaldehyde
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Abcam user community

Verified customer

提交于 Dec 18 2014

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (Breast cancer cell lines)
Specification Breast cancer cell lines
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
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Abcam user community

Verified customer

提交于 Dec 10 2014

Yes this antibody has been tested on human paraffin embedded tissue followed by HIER.

The following publication used our antibody in this application on human placenta:

Lockwood CJ et al. Progestin and thrombin regulate tissue factor ...

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That is no problem at all. I am glad to have been of help.

If I can be of any further assistance, please do let me know.

Until then, I wish you all the best with your experiments.

Thank you for your reply. I am sorry for the delay in getting back to you.

I have attached an image of the staining observed when using the Anti-Vimentin antibody (ab11256) along with the protocol outline used. This is the only image I have fo...

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Thank you for your call today.

I am sorry but following your call I came across the protocol used for paraffin embedded sections with Anti-Vimentin antibody (ab11256). The antigen retrieval was performed using 0.1% Trypsin Enzyme incubation fo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (sertoli cells)
Specification sertoli cells
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
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Dr. Elad Fadlon

Verified customer

提交于 Aug 06 2010

Thank you for your patience. I am sorry to hear that you are experiencing difficulties with this product ab11256 and ab39376 in immunofluorescence. Often it is possible to make suggestions that help resolve problems. We will happily offer technical...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Heart)
Specification Heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Feb 21 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"