The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IHC-P: Ready to use. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Ca(2+)-regulated actin-binding protein.
组织特异性Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
序列相似性Belongs to the villin/gelsolin family. Contains 6 gelsolin-like repeats. Contains 1 HP (headpiece) domain.
结构域Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.