RabMAb

Anti-Villin抗体[EPR3490] (ab109516)

概述

  • 产品名称Anti-Villin抗体[EPR3490]
    参阅全部 Villin 一抗
  • 描述
    兔单克隆抗体[EPR3490] to Villin
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human Villin.

  • 阳性对照
    • Human small intestine, HT-29 and Caco-2 cell lysates; HT-29 cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab109516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 93 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    • 功能Ca(2+)-regulated actin-binding protein.
    • 组织特异性Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
    • 序列相似性Belongs to the villin/gelsolin family.
      Contains 6 gelsolin-like repeats.
      Contains 1 HP (headpiece) domain.
    • 结构域Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.
    • 细胞定位Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 数据库链接
    • 别名
      • D2S1471 antibody
      • OTTHUMP00000164145 antibody
      • VIL antibody
      • VIL1 antibody
      • VILI_HUMAN antibody
      • Villin 1 antibody
      • Villin-1 antibody
      • Villin1 antibody
      see all

    Anti-Villin antibody [EPR3490] 图像

    • All lanes : Anti-Villin antibody [EPR3490] (ab109516) at 1/10000 dilution

      Lane 1 : Human small intestine lysate
      Lane 2 : HT-29 lysate
      Lane 3 : Caco-2 lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 93 kDa
      Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of Villin in permeabilized HT-29 cells, using ab109515 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

    Anti-Villin antibody [EPR3490] (ab109516)参考文献

    This product has been referenced in:
    • Negoro R  et al. Modeling of drug-mediated CYP3A4 induction by using human iPS cell-derived enterocyte-like cells. Biochem Biophys Res Commun 472:631-6 (2016). Flow Cyt ; Human . Read more (PubMed: 26966071) »
    • Ozawa T  et al. Generation of enterocyte-like cells from human induced pluripotent stem cells for drug absorption and metabolism studies in human small intestine. Sci Rep 5:16479 (2015). Flow Cyt ; Human . Read more (PubMed: 26559489) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting us.

    With many products cross reactivity with mouse and rat is determined by western blot only (this is the case for ab109516). If we have only determined the cross reactivity via WB, we will state this information ...

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    Sorry for the confusion. I just wanted to confirm with you that this replacement order, is indeed a free of charge replacement. When the order was first placed the "100%" discount was not taken off the order...

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    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

    Estimated delivery date of this replacement vial is August 27 2012. To ch...

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    Thank you very much for your interest in ab109516. Unfortunately we would not advise that ab109516 be used in ICC. However we have another antibody in our catalogue that may be suitable for your customer. Anti-Villin antibody (ab97512) is a rab...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"