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Synthetic peptide selected from the C-terminal region of Mouse VGLUT2 conjugated to KLH (NP_543129.3).
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Target information above from: UniProt accession
The UniProt ConsortiumThe Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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11 reviews or Q&A
After discussing with my supervisor, I am happy to have a free of charge replacement. Hopefully, I can have a better result. The shipping address is :Thanks,
Asked on Jul 10 2012
Thank you for your email.Could you provide the purchase order number? It is more likely that you have purchased this antibody from our Australian distributor Sapphire Bioscience. Please contact them for purchase order number.Please ...
Abcam Scientific Support
回复于 Jul 10 2012
Thanks for your reply. However, everything you mentioned in the email had been already tried. It could not give the clear band as you assumed. Further, I didnt put two antibodies together. I incubated with two antibodies seperately. I also did no prima...
Asked on Jul 06 2012
Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.The details provided will enable us to investigate this case and will provide us with vital ...
回复于 Jul 06 2012
To answer your questions:1, mouse hippocampus total protein lysate.2, yes, I do added protease when prepare the lysate( roche, cocktail tablet, a tablet per 10ml lysis buffer)3, the tissue used either fresh dissected or properly stored a...
Thank you for your email. I have checked the image and details you have kindly provided and I have following recommendations for troubleshooting;- Heat the lysates at 60-70C for 20 minutes.- Normally, we use BSA for blocking an...
Hello, I used ab84103 to detect vglut2 in the adult mouse hippocampus. I got multiple bands. I even don’t really know which band is the right one. Amount of protein loaded: 10ugBlocking condition: odyssey blocking buffer @4, o...
Asked on Jul 04 2012
Thank you for your email. I am sorry to hear that you have been experiencing problem with this antibody.In order to understand the problem better I have following questions;- How the tissue lysate was prepared?- Did you add pro...
回复于 Jul 04 2012
Inquiry: Hello, I purchased ab84103 for vglut2. I found extra bands besides the band at expected size. I am wondering if any Immunizing Peptide is available for this primary antibody to verify the extra bands as non-specific binding or not? Thank you v...
Asked on Jun 14 2012
Thank you for contacting us.We can provide blocking peptide for this product the price will be £113 for 100ug size. Could you let us know if you are happy purchasing this then I will inform datasheet for publishing the datasheet on webs...
回复于 Jun 14 2012
Hello, 1, Well, I'm not sure if the band I am talking about (indicated by red arrow) is unspecific as it is quite close to the ban around expected size (I indicated by yellow arrow). 2,I was using 8M Urea and 0.1% SDS buffer also containing dTT to resu...
Asked on Dec 15 2011
Thank you for getting back to me and for providing some further details.
Unfortunately, there are no MW bands on the image, so it is hard to assess the size of the two bands observed on the gel. The bands at lower range may well represent some de...
回复于 Dec 15 2011
Hello, I ordered ab84103 a few month ago. Based on my Western blot results, I can see actually two bands (they are quite close to each other) around the expect size. The tissue I used is mouse hippocampus. I am not sure which band is the right one, a...
Thank you for your enquiry regarding ab84103.
It would be much appreciated if I could get some more information which would help me identify the source of the problem.
1) Bands: Could you please specify what size are the non-specific bands? A...
Thanks for your discount, but I am not really sure whether I will do the ICC/IF as I have to have some indication from the WB first and then I can move on. But when I have the results for WB, it might be the early of next year. Is this discount still a...
Asked on Sep 15 2011
I am sorry these are the only products we can recommend. The reason for recommendation is that there is very low sequence homology between the N-terminal and C-terminal end of vglut1 and vglut2. So I am presuming that antibodies against these sequences...
回复于 Sep 15 2011
Thanks for your kind explanation and suggestion. Although ab84103 for vlgut2 seems to be nice antibody, it hasn’t been tested on immunocytochemistry or immunofluroescence. So we don’t know whether this antibody works for those tests. In terms of the ab...
Asked on Sep 14 2011
It is true that ab84103 wasn't tested in ICC/IF however it does not mean, it can't be used. It only means we do not have tested data to confirm it. If you however you would like to use ab84103 in ICC/IF then I can offer you a Abreview testing discount;...
回复于 Sep 14 2011
Thanks for your information. However, I prefer to order ab79157 for vglut2, is there any suggestion of the best antibody I can order for vglut1 to avoid unspecific detection between these two antibodies? Where the sequence do you design the ...
Asked on Sep 13 2011
Thank you for contacting us.
The epitope of ab79157 has not been mapped so we do not know the degree of homology shared by epitope in vGlut1 and vGlut2 proteins.
The predicted band size for vGlu2 protein is 64 kDa. I do agree that there ...
回复于 Sep 13 2011
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"