Anti-VGLUT2抗体[8G9.2] (ab79157)

概述

  • 产品名称Anti-VGLUT2抗体[8G9.2]
    参阅全部 VGLUT2 一抗
  • 描述
    小鼠单克隆抗体[8G9.2] to VGLUT2
  • 经测试应用适用于: IHC-Fr, ICC/IF, IHC-P, WB, IHC-FrFlmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Recombinant full length protein corresponding to Rat VGLUT2.

  • 阳性对照
    • Floating mouse brain tissue sections.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 250mM Sodium chloride, 20mM Sodium phosphate, pH 7.6
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号8G9.2
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 22064485
ICC/IF 1/1000.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 63 kDa (predicted molecular weight: 64 kDa).
IHC-FrFl 1/50 - 1/300.

靶标

  • 功能Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
  • 组织特异性Predominantly expressed in adult brain. Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus.
  • 序列相似性Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
  • 发展阶段Expressed in fetal brain.
  • 细胞定位Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Differentiation associated BNPI antibody
    • Differentiation associated Na dependent inorganic phosphate cotransporter antibody
    • Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody
    • Differentiation-associated BNPI antibody
    • Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody
    • DNPI antibody
    • SLC17A6 antibody
    • Sodium dependent inorganic phosphate cotransporter antibody
    • Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody
    • Solute carrier family 17 member 6 antibody
    • Vesicular glutamate transporter 2 antibody
    • Vesicular glutamate transporter type 2 antibody
    • VGLU2_HUMAN antibody
    • VGLUT 2 antibody
    • VGluT2 antibody
    see all

Anti-VGLUT2 antibody [8G9.2] 图像

  • ab79157, at 1/300 dilution, staining VGLUT2 by Immunohistochemistry using ABC with NiDAB on floating mouse brain tissue sections.
  • Immunocytochemistical detection of VGLUT2 using antibody ab79157 on rat primary hippocampal neuronal culture. Fixative: Paraformaldehyde. Permeabilization: Triton 0.3% in 0.1X PBS. Blocking step: 10% Serum as blocking agent for 20 mins @ 24°C. Primary Antibody: 1 µg/ml for 1 hour @ 24°C in Donkey serum in 0.1%PBS plus Triton 0.3%. Secondary Antibody: Alexa Fluor® 568 Conjugation (1/1000). The submitted image shows ab79157 staining rat primary hippocampal neurons.

  • All lanes : Anti-VGLUT2 antibody [8G9.2] (ab79157) at 1 µg/ml

    Lane 1 : Rat Cortex Tissue Lysate
    Lane 2 : Hippocampus (Mouse) Tissue Lysate
    Lane 3 : Rat Substantia Nigra Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 64 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

Anti-VGLUT2 antibody [8G9.2] (ab79157)参考文献

This product has been referenced in:
  • Wang HS  et al. Changes in VGLUT1 and VGLUT2 expression in rat dorsal root ganglia and spinal cord following spared nerve injury. Neurochem Int 99:9-15 (2016). WB . Read more (PubMed: 27210824) »
  • Hwang HY  et al. TWIK-Related Spinal Cord K? Channel Expression Is Increased in the Spinal Dorsal Horn after Spinal Nerve Ligation. Yonsei Med J 56:1307-15 (2015). IF ; Rat . Read more (PubMed: 26256973) »

See all 9 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Antigen retrieval step None
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.5% Triton X-100
Fixative Formaldehyde
Username

Dr. Hyun Jin Kim

Verified customer

提交于 Mar 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary hippocampal neurons)
Specification Primary hippocampal neurons
Fixative Paraformaldehyde
Permeabilization Yes - Triton 0.3% in 0.1X PBS
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 May 12 2010

Application Immunohistochemistry free floating
Sample Zebra finch Tissue sections (Brain)
Specification Brain
Username

Abcam user community

Verified customer

提交于 Sep 15 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Astatotilapia burtoni Tissue sections (brain)
Permeabilization Yes - 0.1% Triton
Specification brain
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 02 2016

Application Western blot
Sample Rabbit Tissue lysate - whole (brain)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification brain
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 28 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-10% Tris Glycine Gradient gel)
Sample Zebra finch Tissue lysate - whole (whole brain)
Specification whole brain
Blocking step Rotiblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 03 2014

Thank you for your inquiry. The above mentioned antibodies are all tested to be specific for either VGlut1 or VGlut2. The sequence homology is only 73% between the two VGlut proteins and the antibodies are all tested to be specific for either VGlut...

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