重组Anti-VGluT1抗体[EPR10953] - C-terminal (ab180188)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10953] to VGluT1 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-VGluT1抗体[EPR10953] - C-terminal
参阅全部 VGluT1 一抗 -
描述
兔单克隆抗体[EPR10953] to VGluT1 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Fetal brain, Neuro-2a and C6 lysates; ICC/IF: Mouse primary neuron cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR10953 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180188于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (4) |
1/1000 - 1/5000. Predicted molecular weight: 61 kDa.
|
ICC/IF | (1) |
1/200.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 61 kDa. |
ICC/IF
1/200. |
靶标
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功能
Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. -
组织特异性
Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe. -
序列相似性
Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily. -
细胞定位
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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数据库链接
- Entrez Gene: 57030 Human
- Entrez Gene: 72961 Mouse
- Entrez Gene: 116638 Rat
- Omim: 605208 Human
- SwissProt: Q9P2U7 Human
- SwissProt: Q3TXX4 Mouse
- SwissProt: Q62634 Rat
- Unigene: 375616 Human
see all -
别名
- BNPI antibody
- Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
- Brain specific Na dependent inorganic phosphate cotransporter antibody
see all
图片
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling VGluT1 with ab180188 at 1/200 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).
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All lanes : Anti-VGluT1 antibody [EPR10953] - C-terminal (ab180188) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Neuro-2a (Mouse neuroblastoma cell line) lysate
Lane 3 : C6 (Rat glial tumor cell line) lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 61 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab180188 被引用在 8 文献中.
- Triebelhorn J et al. Induced neural progenitor cells and iPS-neurons from major depressive disorder patients show altered bioenergetics and electrophysiological properties. Mol Psychiatry N/A:N/A (2022). PubMed: 35732695
- Verhaeghe R et al. Maternal stress programs a demasculinization of glutamatergic transmission in stress-related brain regions of aged rats. Geroscience N/A:N/A (2021). PubMed: 33983623
- Liu J et al. Infusion of hESC derived Immunity-and-matrix regulatory cells improves cognitive ability in early-stage AD mice. Cell Prolif 54:e13085 (2021). PubMed: 34232542
- Zhou M et al. Quantitative Proteomic Analysis Reveals Synaptic Dysfunction in the Amygdala of Rats Susceptible to Chronic Mild Stress. Neuroscience 376:24-39 (2018). WB . PubMed: 29452226
- Lizarbe B et al. Neurochemical Modifications in the Hippocampus, Cortex and Hypothalamus of Mice Exposed to Long-Term High-Fat Diet. Front Neurosci 12:985 (2018). PubMed: 30670942
- Zhao J et al. APOE e4/e4 diminishes neurotrophic function of human iPSC-derived astrocytes. Hum Mol Genet 26:2690-2700 (2017). PubMed: 28444230
- Zhu C et al. Evidence that CA3 is Underling the Comorbidity Between Pain and Depression and the Co-curation by Wu-Tou decoction in Neuropathic Pain. Sci Rep 7:11935 (2017). PubMed: 28931876
- Lazarczyk MJ et al. Major Histocompatibility Complex class I proteins are critical for maintaining neuronal structural complexity in the aging brain. Sci Rep 6:26199 (2016). WB . PubMed: 27229916