Due to immunogen homology, the antibody may detect VGluT2 (100%) and VGluT3(95%).
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human VGLUT1.
(Peptide available as ab95408.)
- This antibody gave a positive signal in the following Human tissue lysates: Liver; Kidney.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 50 kDa).
Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe.
Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome.
Information by UniProt
- BNPI antibody
- Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
- Brain specific Na dependent inorganic phosphate cotransporter antibody
Western blot - VGLUT1 antibody (ab85922)
All lanes :
Anti-VGluT1 antibody (ab85922) at 1 µg/mlLane 1 :
Human kidney tissue lysate - total protein (ab30203
)Lane 2 :
Human liver tissue lysate - total protein (ab29889
Lysates/proteins at 10 µg per lane.Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique
Performed under reducing conditions.Predicted band size :
50 kDaObserved band size :
49 kDa (why is the actual band size different from the predicted?
)Exposure time :
has not yet been referenced specifically in any publications.
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