概述

  • 产品名称Anti-VGluT1抗体
    参阅全部 VGluT1 一抗
  • 描述
    兔多克隆抗体to VGluT1
  • 特异性Due to immunogen homology, the antibody may detect VGluT2 (100%) and VGluT3(95%).
  • 经测试应用适用于: IHC-Fr, WBmore details
  • 种属反应性
    与反应: Rat, Zebrafish
    预测可用于: Mouse, Cow, Pigeon, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Rat VGluT1.

    (Peptide available as ab93078.)

  • 阳性对照
    • This antibody gave a positive signal in the following rat tissue lysates: Brain; Cerrebellum; Cortex; Forebrain.

性能

应用

Our Abpromise guarantee covers the use of ab77822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/100.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 62 kDa).

靶标

  • 功能Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
  • 组织特异性Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe.
  • 序列相似性Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
  • 细胞定位Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BNPI antibody
    • Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
    • Brain specific Na dependent inorganic phosphate cotransporter antibody
    • Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody
    • Slc17a7 antibody
    • Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody
    • Solute carrier family 17 member 7 antibody
    • Vesicular glutamate transporter 1 antibody
    • VGLU1_HUMAN antibody
    • VGluT1 antibody
    see all

Anti-VGluT1 antibody 图像

  • IHC-Fr image of VGluT1 staining on zebrafish retina sections using ab77822 (1:100). The sections were paraformaldehyde and permeabilized using Triton-X. Antigen retrieval was performed using Tris-HCl and blocking was perfomed using 5% BSA for 1 hour at 23°C. ab77822 was diluted 1:100 and incubated with the sections for 16 hours at 4°C. The secondary antibody was goat polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).

    See Abreview

  • All lanes : Anti-VGluT1 antibody (ab77822) at 1 µg/ml

    Lane 1 : Rat Brain Tissue Lysate
    Lane 2 : Rat Cerebellum Tissue Lysate
    Lane 3 : Rat Cortex Tissue Lysate
    Lane 4 : Rat Forebrain

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 62 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 41 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
    Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

Anti-VGluT1 antibody (ab77822)参考文献

This product has been referenced in:
  • Wang HS  et al. Changes in VGLUT1 and VGLUT2 expression in rat dorsal root ganglia and spinal cord following spared nerve injury. Neurochem Int 99:9-15 (2016). WB . Read more (PubMed: 27210824) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (Brain, axons)
Specification Brain, axons
Permeabilization Yes - Triton X
Fixative Paraformaldehyde
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提交于 Aug 26 2014

DISCOUNT CODES: ab5981 - ******
ab104898 - ***********

Expiration date: 9th July 2012 (for both discount codes)

I am very pleased to hear you would like to accept our offer and test ab5981 and ab104898in hamster tissue]. This ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"