Anti-VGluT1抗体(ab110139)

概述

  • 产品名称Anti-VGluT1抗体
    参阅全部 VGluT1 一抗
  • 描述
    山羊多克隆抗体to VGluT1
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide HDQLAGSDDSEMED(Human)

  • 阳性对照
    • Human brain (cortex) tissue.

性能

应用

Our Abpromise guarantee covers the use of ab110139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
  • 组织特异性Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe.
  • 序列相似性Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
  • 细胞定位Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BNPI antibody
    • Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
    • Brain specific Na dependent inorganic phosphate cotransporter antibody
    • Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody
    • Slc17a7 antibody
    • Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody
    • Solute carrier family 17 member 7 antibody
    • Vesicular glutamate transporter 1 antibody
    • VGLU1_HUMAN antibody
    • VGluT1 antibody
    see all

Anti-VGluT1 antibody 图像

  • ab110139, at 3.75 µg/ml, staining VGluT1 in Human brain (cortex) tissue by immunohistochemistry. After incubation with ab110139, slides were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Anti-VGluT1 antibody (ab110139)参考文献

ab110139 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (synaptosome particles made from human brain)
Permeabilization Yes - tween
Gating Strategy Synaptosomes gated based on size
Specification synaptosome particles made from human brain
Preparation Cell harvesting/tissue preparation method: tissue homogenized and centrifuged to isolate synaptic particles
Sample buffer: PBS
Fixation Paraformaldehyde
Username

Emily Sherfield

Verified customer

提交于 Jun 28 2016

I am sorry these are the only products we can recommend. The reason for recommendation is that there is very low sequence homology between the N-terminal and C-terminal end of vglut1 and vglut2. So I am presuming that antibodies against these sequences...

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It is true that ab84103 wasn't tested in ICC/IF however it does not mean, it can't be used. It only means we do not have tested data to confirm it. If you however you would like to use ab84103 in ICC/IF then I can offer you a Abreview testing discount;...

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Thank you for contacting us. The epitope of ab79157 has not been mapped so we do not know the degree of homology shared by epitope in vGlut1 and vGlut2 proteins. The predicted band size for vGlu2 protein is 64 kDa. I do agree that there ...

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Thank you for contacting us. The N-terminal and C-terminal region amino acids of these proteins do not show sequence homology so the antibodies against the N or C terminal sequence will detect distinct protein they are raised against. Ab...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"