概述

  • 产品名称Anti-VGF抗体
    参阅全部 VGF 一抗
  • 描述
    兔多克隆抗体to VGF
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    17 amino acid peptide near the carboxy terminus of the human VGF (GenBank accession no. NP_003369).

  • 阳性对照
    • Human brain tissue lysate

性能

应用

Our Abpromise guarantee covers the use of ab69989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).

靶标

  • 功能May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.
    NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release.
  • 组织特异性Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.
  • 翻译后修饰Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure.
  • 细胞定位Secreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • NERP-1 antibody
    • NERP-2 antibody
    • Nerve growth factor inducible antibody
    • Neuroendocrine regulatory peptide-2 antibody
    • Neurosecretory protein VGF antibody
    • VGF antibody
    • VGF_HUMAN antibody
    see all

Anti-VGF antibody 图像

  • Lane 1 : Anti-VGF antibody (ab69989) at 0.5 µg/ml
    Lane 2 : Anti-VGF antibody (ab69989) at 1 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 67 kDa
    Observed band size : 67 kDa
  • ab69989 at 5 µg/ml staining VGF in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

  • ICC/IF image of ab69989 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69989, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of VGF in Human Brain cells using ab69989 at 20 ug/ml.

Anti-VGF antibody (ab69989)参考文献

This product has been referenced in:
  • Annaratone L  et al. Search for neuro-endocrine markers (chromogranin A, synaptophysin and VGF) in breast cancers. An integrated approach using immunohistochemistry and gene expression profiling. Endocr Pathol 25:219-28 (2014). WB, IHC-P ; Human . Read more (PubMed: 24277232) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Spermophilus tridecemlineatus Tissue lysate - whole (brain)
Specification brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Apr 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"