The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 67 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
功能May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system. NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release.
组织特异性Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.
翻译后修饰Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure.
细胞定位Secreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.
ab115609, at 5µg/ml, staining VGF in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-VGF antibody (ab115609)
Lane 1 : Anti-VGF antibody (ab115609) at 0.5 µg/ml Lane 2 : Anti-VGF antibody (ab115609) at 1 µg/ml
Lane 1 : Human Brain tissue lysate Lane 2 : Human Brain tissue lysate
Predicted band size : 67 kDa
Anti-VGF antibody (ab115609)参考文献
has not yet been referenced specifically in any publications.