概述

  • 产品名称
    Anti-VGCNL1抗体
  • 描述
    小鼠多克隆抗体to VGCNL1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment: QQQLLSPTLS DRGGSRQDAA DAGKPQRKFG QWRLPSAPKP ISHSVSSVNL RFGGRTTMKS VVCKMNPMTD AASCGSEVKK WWTRQLTVES DESGDDLLDI, corresponding to amino acids 1639-1738 of Human VGCNL1

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度
    Whole antiserum
  • Primary antibody说明
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab43631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 200 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能
    Voltage-independent, cation-nonselective channel which is permeable to sodium, potassium and calcium ions. Responsible for the background sodium ion leak current in neurons and controls neuronal excitability. Activated either by neuropeptides substance P or neurotensin. Required for normal respiratory rhythm and neonatal survival.
  • 疾病相关
    Hypotonia, infantile, with psychomotor retardation and characteristic facies 1
    Congenital contractures of the limbs and face, hypotonia, and developmental delay
  • 序列相似性
    Belongs to the cation-nonselective channel family.
  • 结构域
    Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A530023G15Rik antibody
    • bA430M15.1 antibody
    • CanIon antibody
    • Canlon antibody
    • FLJ23913 antibody
    • FLJ44659 antibody
    • FLJ44764 antibody
    • Four repeat voltage gated ion channel antibody
    • MGC74524 antibody
    • Nalcn antibody
    • NALCN_HUMAN antibody
    • Putative 4 repeat voltage gated ion channel antibody
    • Sodium leak channel non-selective protein antibody
    • sodium leak channel, non selective antibody
    • Vgcnl1 antibody
    • VGCNL1 protein antibody
    • voltage gated channel like 1 antibody
    • Voltage gated channel-like protein 1 antibody
    see all

实验方案

文献

ab43631 has not yet been referenced specifically in any publications.

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