概述
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产品名称Anti-Vesicular Acetylcholine Transporter抗体
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描述羊多克隆抗体to Vesicular Acetylcholine Transporter
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特异性Ab31544 is specific to vesicular acetylcholine transporter.
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经测试应用适用于: IHC - Wholemount, WBmore details
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种属反应性与反应: Rat, Guinea pig
预测可用于: Mouse, Dog, Human, Chimpanzee, Macaque monkey
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免疫原
Synthetic peptide:
C-TRSRSERDVLLDEPPQGLYDAVRLRE
, corresponding to amino acids 475-500 of Rat Vesicular Acetylcholine Transporter conjugated to Blue carrier protein.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: None
Constituents: Whole serum -
纯度Whole antiserum
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克隆多克隆
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同种型IgG
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研究领域
应用
Our Abpromise guarantee covers the use of ab31544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC - Wholemount | Use at an assay dependent concentration. Fix Tissue overnight in Zamboni fixative, then carry out 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS and Azide until being dissected and stained. | |
| WB | Use at an assay dependent dilution. Predicted molecular weight: 59 kDa. |
靶标
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功能Involved in acetylcholine transport into synaptic vesicles.
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组织特异性Peripheral and central cholinergic nervous systems.
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序列相似性Belongs to the major facilitator superfamily. Vesicular transporter family.
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细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 6572 Human
- Entrez Gene: 20508 Mouse
- Entrez Gene: 60422 Rat
- Omim: 600336 Human
- SwissProt: Q16572 Human
- SwissProt: O35304 Mouse
- SwissProt: Q62666 Rat
- Unigene: 654374 Human
see all -
别名
- AChR antibody
- MGC12716 antibody
- rVAT antibody
see all
Anti-Vesicular Acetylcholine Transporter antibody 图像
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Immunohistochemistry - Anti-Vesicular Acetylcholine Transporter antibody (ab31544)Immunohistochemical analysis of Vesicular Acetylcholine Transporter in guinea pig gut.
Method: Guinea Pig Ileum removed and fixed overnight in Zamboni fixative, then cleared 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS Azide until being dissected and used in this experiment.
Primary: Anti-Vesicular Acetylcholine Transporter antibody (ab31544) diluted 1:1000 incubated overnight, washed 3 x 10 mins.
Secondary: antibody incubated for 2 hours washed in PBS, put in 100% Glycerol and mounted.
Magnification: 40X.
Anti-Vesicular Acetylcholine Transporter antibody (ab31544)参考文献
ab31544 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"