Anti-Vesicular Acetylcholine Transporter抗体(ab31544)

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概述

  • 产品名称
    Anti-Vesicular Acetylcholine Transporter抗体
  • 描述
    羊多克隆抗体to Vesicular Acetylcholine Transporter
  • 宿主
    Sheep
  • 特异性
    Ab31544 is specific to vesicular acetylcholine transporter.
  • 经测试应用
    适用于: IHC - Wholemount, WBmore details
  • 种属反应性
    与反应: Rat, Guinea pig
    预测可用于: Mouse, Dog, Human, Chimpanzee, Macaque monkey
  • 免疫原

    Synthetic peptide:

    C-TRSRSERDVLLDEPPQGLYDAVRLRE

    , corresponding to amino acids 475-500 of Rat Vesicular Acetylcholine Transporter conjugated to Blue carrier protein.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

性能

应用

Our Abpromise guarantee covers the use of ab31544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC - Wholemount Use at an assay dependent concentration. Fix Tissue overnight in Zamboni fixative, then carry out 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS and Azide until being dissected and stained.
WB Use at an assay dependent dilution. Predicted molecular weight: 59 kDa.

靶标

图片

  • Immunohistochemistry - Anti-Vesicular Acetylcholine Transporter antibody (ab31544)
    Immunohistochemistry - Anti-Vesicular Acetylcholine Transporter antibody (ab31544)
    Immunohistochemical analysis of Vesicular Acetylcholine Transporter in guinea pig gut.

    Method: Guinea Pig Ileum removed and fixed overnight in Zamboni fixative, then cleared 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS Azide until being dissected and used in this experiment.
    Primary: Anti-Vesicular Acetylcholine Transporter antibody (ab31544) diluted 1:1000 incubated overnight, washed 3 x 10 mins.
    Secondary: antibody incubated for 2 hours washed in PBS, put in 100% Glycerol and mounted.
    Magnification: 40X.

文献

ab31544 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab31544？请让我们知道，以便我们可以引用本数据表中的参考文章。

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vesicular Acetylcholine Transporter antibody

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Tween 20
Specification
Spinal cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
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Mrs. Teresa Garrett

Verified customer

提交于 Oct 14 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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