Anti-Vesicular Acetylcholine Transporter抗体(ab31544)

概述

  • 产品名称Anti-Vesicular Acetylcholine Transporter抗体
    参阅全部 Vesicular Acetylcholine Transporter 一抗
  • 描述
    羊多克隆抗体to Vesicular Acetylcholine Transporter
  • 特异性Ab31544 is specific to vesicular acetylcholine transporter.
  • 经测试应用适用于: IHC - Wholemount, WBmore details
  • 种属反应性
    与反应: Rat, Guinea pig
    预测可用于: Mouse, Dog, Human, Chimpanzee, Macaque Monkey
  • 免疫原

    Synthetic peptide:

    C-TRSRSERDVLLDEPPQGLYDAVRLRE

    , corresponding to amino acids 475-500 of Rat Vesicular Acetylcholine Transporter conjugated to Blue carrier protein.

性能

应用

Our Abpromise guarantee covers the use of ab31544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC - Wholemount Use at an assay dependent concentration. Fix Tissue overnight in Zamboni fixative, then carry out 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS and Azide until being dissected and stained.
WB Use at an assay dependent dilution. Predicted molecular weight: 59 kDa.

靶标

Anti-Vesicular Acetylcholine Transporter antibody 图像

  • Immunohistochemical analysis of Vesicular Acetylcholine Transporter in guinea pig gut.

    Method: Guinea Pig Ileum removed and fixed overnight in Zamboni fixative, then cleared 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS Azide until being dissected and used in this experiment.
    Primary: Anti-Vesicular Acetylcholine Transporter antibody (ab31544) diluted 1:1000 incubated overnight, washed 3 x 10 mins.
    Secondary: antibody incubated for 2 hours washed in PBS, put in 100% Glycerol and mounted.
    Magnification: 40X.

Anti-Vesicular Acetylcholine Transporter antibody (ab31544)参考文献

ab31544 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Spinal cord)
Antigen retrieval step None
Permeabilization Yes - 0.1% Tween 20
Specification Spinal cord
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
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Mrs. Teresa Garrett

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提交于 Oct 14 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"