The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC - Wholemount
Use at an assay dependent concentration. Fix Tissue overnight in Zamboni fixative, then carry out 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS and Azide until being dissected and stained.
Use at an assay dependent dilution. Predicted molecular weight: 59 kDa.
Involved in acetylcholine transport into synaptic vesicles.
Peripheral and central cholinergic nervous systems.
Belongs to the major facilitator superfamily. Vesicular transporter family.
Immunohistochemical analysis of Vesicular Acetylcholine Transporter in guinea pig gut.
Method: Guinea Pig Ileum removed and fixed overnight in Zamboni fixative, then cleared 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS Azide until being dissected and used in this experiment. Primary: Anti-Vesicular Acetylcholine Transporter antibody (ab31544) diluted 1:1000 incubated overnight, washed 3 x 10 mins. Secondary: antibody incubated for 2 hours washed in PBS, put in 100% Glycerol and mounted. Magnification: 40X.