Synthetic peptide corresponding to Mouse VEGFR 1+VEGFR 2 aa 800-900.
Sequence:
PDEVPLDEQCERLPYDASKWEFARERLKLGKSLGRGAFGK VVQASAFG IKKSPTCRTVAVKMLKEGATASEYKALMTELKILTHIGHHLNVVNLLGAC TK
Our Abpromise guarantee covers the use of ab36844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/100 - 1/2000. Predicted molecular weight: 150 kDa. | |
ICC/IF | 1/500. | |
IHC-P | 1/250 - 1/500. |
IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:500 dilution of ab36844. The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes.
IHC analysis of a formalin-fixed paraffin-embedded (FFPE) human breast carcinoma tissue section using 1:500 dilution of ab36844. The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes.
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