Anti-VEGFD抗体(ab103685)

概述

  • 产品名称Anti-VEGFD抗体
    参阅全部 VEGFD 一抗
  • 描述
    兔多克隆抗体to VEGFD
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human VEGFD.

  • 阳性对照
    • This antibody gave a positive signal in HT1080 whole cell lysate as well as the following tissue lysates: Human Heart; Human Fetal Lung; Human Colon.

性能

应用

Our Abpromise guarantee covers the use of ab103685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P Use a concentration of 10 µg/ml.

靶标

  • 功能Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
  • 组织特异性Highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas.
  • 序列相似性Belongs to the PDGF/VEGF growth factor family.
  • 翻译后修饰Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3 and VEGFR-2. VEGF-D first form an antiparallel homodimer linked by disulfide bonds before secretion. The fully processed VEGF-D is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • c-fos induced growth factor antibody
    • c-fos induced growth factor (vascular endothelial growth factor D) antibody
    • c-fos induced growth factor antibody
    • c-fos-induced growth factor antibody
    • FIGF antibody
    • Vascular endothelial growth factor D antibody
    • Vascular endothelial growth factor D precursor antibody
    • Vascular endothelial growth factor D precursor antibody
    • VEGF D antibody
    • VEGF-D antibody
    • VEGFD antibody
    • VEGFD_HUMAN antibody
    see all

Anti-VEGFD antibody 图像

  • All lanes : Anti-VEGFD antibody (ab103685) at 1 µg/ml

    Lane 1 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human Fetal Heart - Total Protein Tissue Lysate
    Lane 4 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 140 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • IHC image of VEGFD staining in Human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab103685, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-VEGFD antibody (ab103685)参考文献

ab103685 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.
We are happy to reassure our customers that all of our products are covered by our Abpromise, which gu...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"