概述

  • 产品名称Anti-VEGFB抗体
    参阅全部 VEGFB 一抗
  • 描述
    兔多克隆抗体to VEGFB
  • 特异性ab58461 detects endogenous levels of total VEGFB protein.
  • 经测试应用适用于: WB, ELISA, Sandwich ELISAmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from internal sequence of human VEGFB.

  • 阳性对照
    • Extracts from Jurkat cells

性能

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应用

Our Abpromise guarantee covers the use of ab58461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA 1/20000.
Sandwich ELISA Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab76863 as Capture.

靶标

  • 功能Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
  • 组织特异性Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
  • 序列相似性Belongs to the PDGF/VEGF growth factor family.
  • 翻译后修饰VEGF-B186 is O-glycosylated.
  • 细胞定位Secreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
  • Information by UniProt
  • 数据库链接
  • 形式There are 2 isoforms produced by alternative splicing.
  • 别名
    • Vascular endothelial growth factor B antibody
    • VEGF B antibody
    • VEGF related factor antibody
    • VEGF-B antibody
    • VEGF-related factor antibody
    • Vegfb antibody
    • VEGFB_HUMAN antibody
    • VEGFL antibody
    • VRF antibody
    see all

Anti-VEGFB antibody 图像

  • Standard curve for VEGFB (Analyte: ab87052); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse polyclonal to VEGFB (ab76863) at 5µg/ml and Detector Antibody Rabbit polyclonal to VEGFB (ab58461) at 0.5µg/ml.
  • All lanes : Anti-VEGFB antibody (ab58461) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with immunizing peptide


    Predicted band size : 22 kDa
    Observed band size : 22 kDa

实验方案

Anti-VEGFB antibody (ab58461)参考文献

This product has been referenced in:
  • Guan W  et al. Vascular protection by Angiotensin receptor antagonism involves differential VEGF expression in both hemispheres after experimental stroke. PLoS One 6:e24551 (2011). WB ; Rat . Read more (PubMed: 21912702) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (hippocampus)
Gel Running Conditions Reduced Denaturing (12%gel)
Loading amount 20 µg
Specification hippocampus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Abcam user community

Verified customer

提交于 Apr 29 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (cardiomyocytes)
Loading amount 30 µg
Specification cardiomyocytes
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jul 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"