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Our Abpromise guarantee covers the use of ab27620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Immunohistochemical staining of human angiosarcoma labelling VEGF (SP28) with ab27620 at a 1:1 no dilution.