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Our Abpromise guarantee covers the use of ab28775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
ab28775 staining VEGFA in Human shoulder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/50) for 24 hours at 4°C. A Biotin-conjugated Horse anti-mouse polyclonal (1/1000) was used as the secondary antibody
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