Anti-VEGFA抗体(Biotin) (ab84994)

概述

  • 产品名称Anti-VEGFA抗体(Biotin)
    参阅全部 VEGFA 一抗
  • 描述
    兔多克隆抗体to VEGFA (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: ELISA, Sandwich ELISA, WBmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Highly pure (>98%) recombinant Rat VEGF

性能

应用

Our Abpromise guarantee covers the use of ab84994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
Sandwich ELISA
WB
  • 应用说明ELISA: Use at a concentration of 0.25 - 1 µg/ml. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat VEGF.

    sELISA: Use at a concentration of 0.25 - 1 µg/ml. This biotinylated polyclonal antibody, in conjunction with ab70612 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat VEGF

    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 27 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
    • 组织特异性Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
    • 疾病相关Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
    • 序列相似性Belongs to the PDGF/VEGF growth factor family.
    • 细胞定位Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
    • Information by UniProt
    • 数据库链接
    • 别名
      • MGC70609 antibody
      • MVCD1 antibody
      • Vascular endothelial growth factor A antibody
      • vascular endothelial growth factor antibody
      • Vascular permeability factor antibody
      • VEGF A antibody
      • Vegf antibody
      • VEGF-A antibody
      • VEGF120 antibody
      • Vegfa antibody
      • VEGFA_HUMAN antibody
      • VPF antibody
      see all

    Anti-VEGFA antibody (Biotin) (ab84994)参考文献

    ab84994 has not yet been referenced specifically in any publications.

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    We do actually recommend storing the lyophilised antibody at -20C for long term storage; this will be stable for 2 years.

    The antibody should be stored lyophilised at -20C till use and then reconstituted...

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