概述

性能

应用

Our Abpromise guarantee covers the use of ab9570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGFA (Biotin) (ab83132).

To detect hVEGF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody as Detection is required. This antigen affinity purified antibody, paired with the above recommended pair, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF (ab9571).

ELISA Use at an assay dependent concentration. To detect hVEGF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hVEGF.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hVEGF (10.0 ng/ml), a concentration of 0.05 - 0.1 µg/ml of this antibody is required.
WB Use a concentration of 0.1 - 0.2 µg/ml.
IHC-P Use a concentration of 2.5 - 5.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • 组织特异性Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • 疾病相关Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 序列相似性Belongs to the PDGF/VEGF growth factor family.
  • 细胞定位Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC70609 antibody
    • MVCD1 antibody
    • Vascular endothelial growth factor A antibody
    • vascular endothelial growth factor antibody
    • Vascular permeability factor antibody
    • VEGF A antibody
    • Vegf antibody
    • VEGF-A antibody
    • VEGF120 antibody
    • Vegfa antibody
    • VEGFA_HUMAN antibody
    • VPF antibody
    see all

Anti-VEGFA antibody 图像

  • ab9570 staining VEGF in human breast invasive ductal carcinoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 2.5 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

  • Lane 1 :
    Lanes 2 - 12 : Anti-VEGFA antibody (ab9570) at 0.2 µg/ml

    Lane 1 : MultiMark MultiColor Standard
    Lane 2 : Human VEGF at 0.25 µg
    Lane 3 : Human VEGF at 0.125 µg
    Lane 4 : Human VEGF at 0.0625 µg
    Lane 5 : Human VEGF at 0.03125 µg
    Lane 6 : Human VEGF at 0.015625 µg
    Lane 7 : Human VEGF at 0.0078 µg
    Lane 8 : Human VEGF at 0.0039 µg
    Lane 9 : Human VEGF at 0.00195 µg
    Lane 10 : Human VEGF at 0.000975 µg
    Lane 11 : Human VEGF at 0.0004875 µg
    Lane 12 : Human VEGF at 0.00024 µg


    Observed band size : 38.2 kDa (why is the actual band size different from the predicted?)
  • Detected human VEGF in a sandwich ELISA within the range 16-1000 pg/ml
    - 0.5 µg/ml of capture antibody (ab9570)
    - 0.25 µg/ml of detection antibody (ab83132)

Anti-VEGFA antibody (ab9570)参考文献

This product has been referenced in:
  • Banerjee P  et al. Identification of key contributory factors responsible for vascular dysfunction in idiopathic recurrent spontaneous miscarriage. PLoS One 8:e80940 (2013). ELISA ; Human . Read more (PubMed: 24260517) »

See 1 Publication for this product

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I just wanted to let you know that the protein has been ordered and you will be receiving this free of charge during the first week of July.

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This product is sold in a 100ug size at a concentration of 0.5mg/ml. Therefore each vial should contain 200ul.

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I have had the lab do some further investigation with this antibody.We found one that was run reduced rather than the unreduced one I previously sent. We have spoken with the antibody lab to confirm, but ...

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Thank you for contacting Abcam regarding ab9570.


I am sorry to hear you have been obtaining some confusing results with this antibody. I have checked with the lab and the same isoform of VEGF that you have purchased was also used as the...

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For the proteins, I would suggest using ab61861 as this protein is arecombinant Human VEGF 165A protein (double, non-glycosylated, polypeptide chain containing 165 amino acids, MW 38kDa, expressed in E.coli). Read More

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Thefollowing CROSS REACTIVITY has been tested with the antibodies ab9570 and ab83132:

When prepared at 50ng/ml the following antigens exhibited 100% cross reactivity:

Human VEGF121
Murin...

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The WB protocol is as follows:

1. After the protein has been transferred to the nitrocellulose membrane, incubate it in a blocking solution containing 3% Nonfat Dry Milk. The membrane should b...

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Thank you for contacting us. Unless we specify it on our datasheet, all the antibodies work under denatured, reducing conditions. However, this antibody should work under both reduced and non-reduced conditions. I would suggest repeating the assay usin...

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