概述

  • 产品名称
  • 描述
    兔多克隆抗体to VEGFA
  • 经测试应用
    适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein corresponding to Mouse VEGFA. Recombinant mouse VEGF164
    Database link: Q00731

  • 阳性对照
    • SaOS2 cells. Human pancreas tissue.

性能

应用

Our Abpromise guarantee covers the use of ab51745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use a concentration of 1 - 10 µg/ml.
IHC-P Use a concentration of 2 - 10 µg/ml.

靶标

  • 功能
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • 组织特异性
    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • 疾病相关
    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 序列相似性
    Belongs to the PDGF/VEGF growth factor family.
  • 细胞定位
    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Folliculostellate cell-derived growth factor antibody
    • Glioma-derived endothelial cell mitogen antibody
    • MGC70609 antibody
    • MVCD1 antibody
    • Vascular endothelial growth factor A antibody
    • vascular endothelial growth factor A121 antibody
    • vascular endothelial growth factor A165 antibody
    • vascular endothelial growth factor antibody
    • Vascular permeability factor antibody
    • VEGF A antibody
    • Vegf antibody
    • VEGF-A antibody
    • VEGF120 antibody
    • Vegfa antibody
    • VEGFA_HUMAN antibody
    • VPF antibody
    see all

Anti-VEGFA antibody 图像

  • Anti-VEGFA antibody (ab51745) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 61 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes VEGFA contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Staining of VEGF-A in paraffin-embedded mouse ventricular zone of the CNS (E17) and a perinecrotic area of a human glioblastom.

    Top panel: Mouse E17: ventricular zone of the CNS.

    Bottom panel: Human glioblastoma: perinecrotic area.

    The experiments were performed by Dr. Till Acker and Prof. K.H. Plate, Neurological Institute, Neuropathology, Deutschordenstr. 45, 60528 Frankfurt, Germany

  • Sandwich-ELISA using ab51745 VEGFA as capture antibody. A biotinylated polyclonal anti-mouse VEGFA antibody was used for detection and recombinant human VEGF165 and mouse VEGF164 as standards.

Anti-VEGFA antibody (ab51745)参考文献

This product has been referenced in:
  • Li Z  et al. Ferulic Acid Improves Functional Recovery after Acute Spinal Cord Injury in Rats by Inducing Hypoxia to Inhibit microRNA-590 and Elevate Vascular Endothelial Growth Factor Expressions. Front Mol Neurosci 10:183 (2017). WB ; Rat . Read more (PubMed: 28642684) »
  • Yang X  et al. DPPIV promotes endometrial carcinoma cell proliferation, invasion and tumorigenesis. Oncotarget 8:8679-8692 (2017). WB . Read more (PubMed: 28060721) »

See all 17 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Tumor tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH8.0
Permeabilization
No
Specification
Tumor tissue
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 50µg/mL · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 23 2015

Thank you for contacting us.


The rabbit anti-mouse VEGF-A antibody ab51745 did not cross react with human VEGF-C in Western blotting (reduced, non-reduced). According to our results the rabbit anti-rat VEGF-C ab9546 cross reacts with huma...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Melanoma cancer)
Loading amount
30 µg
Specification
Melanoma cancer
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

提交于 Jan 02 2013

Thank you for your enquiry. The MW of recombinant mouse VEGF164 dimer produced in insect cells is about 48 kDa, but the monomer has a MW of about 24 kDa.. The predicted size of 27 kDa is probably those of VEGF164 produced in mammalian cells. This i...

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