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Our Abpromise guarantee covers the use of ab51745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.|
|ELISA||Use a concentration of 1 - 10 µg/ml.|
|IHC-P||Use a concentration of 2 - 10 µg/ml.|
Staining of VEGF-A in paraffin-embedded mouse ventricular zone of the CNS (E17) and a perinecrotic area of a human glioblastom.
Top panel: Mouse E17: ventricular zone of the CNS.
Bottom panel: Human glioblastoma: perinecrotic area.
The experiments were performed by Dr. Till Acker and Prof. K.H. Plate, Neurological Institute, Neuropathology, Deutschordenstr. 45, 60528 Frankfurt, Germany
Sandwich-ELISA using ab51745 VEGFA as capture antibody. A biotinylated polyclonal anti-mouse VEGFA antibody was used for detection and recombinant human VEGF165 and mouse VEGF164 as standards.