概述

  • 产品名称Anti-VEGFA抗体
    参阅全部 VEGFA 一抗
  • 描述
    兔多克隆抗体to VEGFA
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein corresponding to Mouse VEGFA. Recombinant mouse VEGF164
    Database link: Q00731

  • 阳性对照
    • SaOS2 cells. Human pancreas tissue.

性能

应用

Our Abpromise guarantee covers the use of ab51745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant murine VEGF-A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use a concentration of 1 - 10 µg/ml.
IHC-P Use a concentration of 2 - 10 µg/ml.

靶标

  • 功能Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • 组织特异性Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • 疾病相关Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 序列相似性Belongs to the PDGF/VEGF growth factor family.
  • 细胞定位Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC70609 antibody
    • MVCD1 antibody
    • Vascular endothelial growth factor A antibody
    • vascular endothelial growth factor antibody
    • Vascular permeability factor antibody
    • VEGF A antibody
    • Vegf antibody
    • VEGF-A antibody
    • VEGF120 antibody
    • Vegfa antibody
    • VEGFA_HUMAN antibody
    • VPF antibody
    see all

Anti-VEGFA antibody 图像

  • Anti-VEGFA antibody (ab51745) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 61 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
    VEGFA contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Staining of VEGF-A in paraffin-embedded mouse ventricular zone of the CNS (E17) and a perinecrotic area of a human glioblastom.

    Top panel: Mouse E17: ventricular zone of the CNS.

    Bottom panel: Human glioblastoma: perinecrotic area.

    The experiments were performed by Dr. Till Acker and Prof. K.H. Plate, Neurological Institute, Neuropathology, Deutschordenstr. 45, 60528 Frankfurt, Germany

  • Sandwich-ELISA using ab51745 VEGFA as capture antibody. A biotinylated polyclonal anti-mouse VEGFA antibody was used for detection and recombinant human VEGF165 and mouse VEGF164 as standards.

Anti-VEGFA antibody (ab51745)参考文献

This product has been referenced in:
  • Tang J  et al. The role of the AMOP domain in MUC4/Y-promoted tumour angiogenesis and metastasis in pancreatic cancer. J Exp Clin Cancer Res 35:91 (2016). WB ; Human . Read more (PubMed: 27287498) »
  • Wang L  et al. miR-126 Regulation of Angiogenesis in Age-Related Macular Degeneration in CNV Mouse Model. Int J Mol Sci 17:N/A (2016). WB ; Mouse . Read more (PubMed: 27338342) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Tumor tissue)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH8.0
Permeabilization No
Specification Tumor tissue
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 50µg/mL · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 23 2015

Thank you for contacting us.


The rabbit anti-mouse VEGF-A antibody ab51745 did not cross react with human VEGF-C in Western blotting (reduced, non-reduced). According to our results the rabbit anti-rat VEGF-C ab9546 cross reacts with huma...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Melanoma cancer)
Loading amount 30 µg
Specification Melanoma cancer
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Ms. Seontae Kim

Verified customer

提交于 Jan 02 2013

Thank you for your enquiry. The MW of recombinant mouse VEGF164 dimer produced in insect cells is about 48 kDa, but the monomer has a MW of about 24 kDa.. The predicted size of 27 kDa is probably those of VEGF164 produced in mammalian cells. This i...

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