Anti-VEGF Receptor 3抗体(ab27278)

概述

  • 产品名称Anti-VEGF Receptor 3抗体
    参阅全部 VEGF Receptor 3 一抗
  • 描述
    兔多克隆抗体to VEGF Receptor 3
  • 经测试应用适用于: IHC - Wholemount, ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    LASEEFEQIESRHRQESGFR

    , corresponding to C terminal amino acids 1279-1298 of Human VEGF Receptor 3

  • 阳性对照
    • Human placenta or breast carcinoma for Immunohistochemistry.

性能

应用

Our Abpromise guarantee covers the use of ab27278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC - Wholemount 1/200.
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 146 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Receptor for VEGFC. Has a tyrosine-protein kinase activity.
  • 组织特异性Placenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain.
  • 疾病相关Defects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
    Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 2.7.10.1 antibody
    • flt 4 antibody
    • FLT-4 antibody
    • FLT4 antibody
    • FLT41 antibody
    • Fms related tyrosine kinase 4 antibody
    • Fms-like tyrosine kinase 4 antibody
    • LMPH1A antibody
    • PCL antibody
    • Soluble VEGFR3 variant 1 antibody
    • Soluble VEGFR3 variant 2 antibody
    • Soluble VEGFR3 variant 3 antibody
    • Tyrosine protein kinase receptor FLT4 antibody
    • Tyrosine-protein kinase receptor FLT4 antibody
    • Vascular endothelial growth factor receptor 3 antibody
    • Vascular endothelial growth factor receptor 3 precursor antibody
    • VEGF R3 antibody
    • VEGFR 3 antibody
    • VEGFR-3 antibody
    • VEGFR3 antibody
    • VGFR3_HUMAN antibody
    see all

Anti-VEGF Receptor 3 antibody 图像

  • Ab27278 at a dilution of 1/100, staining formalin fixed paraffin embedded Flt4 in human placenta by Immunohistochemistry.
  • ICC/IF image of ab27278 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27278, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-VEGF Receptor 3 antibody (ab27278) at 1/50 dilution

    Predicted band size : 146 kDa
    Observed band size : 170,200 kDa (why is the actual band size different from the predicted?)
    Hey cell lysate (50µg/lane) was used. The antibody was diluted at 1:50 for 2 hours at room tepterature Hey cell lysate (50µg/lane) was used. The antibody was diluted at 1:50 for 2 hours at room temperature. The protein is heavily glycosylated and this might explain the increase in MW above the predcted MW as well as the multiple bands.

Anti-VEGF Receptor 3 antibody (ab27278)参考文献

This product has been referenced in:
  • Yue T  et al. Interleukin 12 shows a better curative effect on lung cancer than paclitaxel and cisplatin doublet chemotherapy. BMC Cancer 16:665 (2016). IHC-Fr ; Mouse . Read more (PubMed: 27549240) »
  • Naruse T  et al. Immunohistochemical study of vascular endothelial growth factor-C/vascular endothelial growth factor receptor-3 expression in oral tongue squamous cell carcinoma: Correlation with the induction of lymphangiogenesis. Oncol Lett 10:2027-2034 (2015). IHC-P ; Human . Read more (PubMed: 26622791) »

See all 12 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (PLACENTA)
Antigen retrieval step Enzymatic
Permeabilization No
Specification PLACENTA
Blocking step Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 22 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Skin)
Specification Skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Dec 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application IHC - Wholemount
Sample Rat Tissue (brain)
Specification brain
Username

Xiao Mao

Verified customer

提交于 Dec 17 2012

I am very pleased to hear you would like to accept our offer and test ab27278 in rat. This code will give you the VALUE of this product off an order before the expiration date. To redeem this offer, please submit an Abreview for rat. The code is inacti...

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Thank you for contacting us. I have created a new 100% Abreview Testing Discount Code for testing AB27278 in Porcine. This code will expire on xxxxxxx. xxxxx I hope this information is helpful to you. Please do not hesitate to contact us if yo...

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Thank you very much for your interest in 27278.

To our knowledge, ab27278 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab27278 now, test it in IHC-Fr and submit feedback to us in the form of...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cow Tissue sections (Ovary)
Specification Ovary
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization No
Blocking step Serum as blocking agent for 40 minute(s) · Concentration: 2.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jul 31 2012

Application Western blot
Sample Pig Tissue lysate - whole (Pig heart (left ventricle))
Loading amount 50 µg
Specification Pig heart (left ventricle)
Gel Running Conditions Reduced Denaturing (3-8% Novex Tris-Acetate)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Apr 09 2012

I am very pleased to hear you would like to accept our offer and test ab27278 in porcine tissues. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for porcine tissues and includ...

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I would be comfortable recommending this product for a number of reasons. The amino acid changes are minimal, the polarities remain the same and the hydropathy of the amino acids are similar. There should be minimal or no issues from this.

T...

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