Anti-VEGF Receptor 2 (phospho Y1054 + Y1059)抗体(ab5473)


  • 产品名称
    Anti-VEGF Receptor 2 (phospho Y1054 + Y1059)抗体
    参阅全部 VEGF Receptor 2 一抗
  • 描述
    兔多克隆抗体to VEGF Receptor 2 (phospho Y1054 + Y1059)
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phospho peptide (Human)containing Tyrosines 1054 and 1059. This sequence is conserved in mouse and rat.

  • 阳性对照
    • Porcine aortic endothelial cells transfected with a chimeric receptor consisting of the extracellular portion of the CSF 1 receptor fused with the transmembrane and intracellular domains of mouse VEGFR 2.



Our Abpromise guarantee covers the use of ab5473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 20566746
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 200 kDa.


  • 功能
    Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • 疾病相关
    Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • 细胞定位
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD309 antibody
    • CD309 antigen antibody
    • EC antibody
    • Fetal liver kinase 1 antibody
    • FLK-1 antibody
    • FLK1 antibody
    • FLK1, mouse, homolog of antibody
    • Kdr antibody
    • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
    • Kinase insert domain receptor antibody
    • KRD1 antibody
    • Ly73 antibody
    • Protein tyrosine kinase receptor FLK1 antibody
    • Protein-tyrosine kinase receptor flk-1 antibody
    • soluble VEGFR2 antibody
    • Tyrosine kinase growth factor receptor antibody
    • Vascular endothelial growth factor receptor 2 antibody
    • VEGFR 2 antibody
    • VEGFR antibody
    • VEGFR-2 antibody
    • VEGFR2 antibody
    • VGFR2_HUMAN antibody
    see all

Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) antibody 图像

  • All lanes : Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) antibody (ab5473) at 1/100 dilution

    Lane 1 : Human umbilical vein endothelial cells with Skimmed milk
    Lane 2 : Human umbilical vein endothelial cells treated with 25ng/ml VEGF with Skimmed milk
    Lane 3 : MDA-MB-231 with Skimmed milk
    Lane 4 : MCF7 with Skimmed milk

    Lysates/proteins at 30 µg per lane.
    Blocking peptides at 5 % per lane.

    HRP conjugated Goat anti-rabbit IgG at 1/5000 dilution
  • ab5473 staining VEGF Receptor 2 (phospho Y1054 + Y1059) in 90% confluent log phase Human umbilical vein endothelial cells (panel a) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (2 ug/ml in 0.1% BSA) for 3 hours at room temperature. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/2000). Nuclei stained blue with DAPI (panel b), F-actin stained red with Alexa Fluor® 555 Rhodamine Phalloidin (panel c), merged image shown in panel d.

  • Peptide Competition and Stimulation: Extracts prepared from PAE cells transfected with a chimeric receptor (see Positive Controls section above for full description) were left unstimulated (1, 2) or stimulated with VEGF (3-8), then were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with a VEGFR2 pan antibody (1, 3) or 0.50 µg/mL VEGFR2 ab5473 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (2, 4, 5), the non-phosphopeptide corresponding to the immunogen (6), a generic phosphotyrosine containing peptide (7), or, the phosphopeptide immunogen (8). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5473 blocks the antibody signal,

Anti-VEGF Receptor 2 (phospho Y1054 + Y1059) antibody (ab5473)参考文献

This product has been referenced in:
  • Reeson P  et al. Delayed inhibition of VEGF signaling after stroke attenuates blood-brain barrier breakdown and improves functional recovery in a comorbidity-dependent manner. J Neurosci 35:5128-43 (2015). WB ; Mouse . Read more (PubMed: 25834040) »
  • Dai J  et al. Cabozantinib inhibits prostate cancer growth and prevents tumor-induced bone lesions. Clin Cancer Res 20:617-30 (2014). Human . Read more (PubMed: 24097861) »

See all 10 Publications for this product

Product Wall

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6.0
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Mr. Mc Shen

Verified customer

提交于 Dec 20 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate bufer
Rat Tissue sections (rat bone marrow tissue)
rat bone marrow tissue

Abcam user community

Verified customer

提交于 Mar 07 2014

Thank you for contacting Abcam regarding expiration dates of our products.

At Abcam, we guarantee the effectiveness of our products for a period of 6 months from date of delivery. Should you experience any issues using one of our products in...

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Thank you for your enquiry. I can confirm that the immunogen sequence used to generate this polyclonal antibody was the dual phosphorylated form of VEGF receptor 2. (pYpY1054/1057). Because of the nature of polyclonal antibodies , it is potentially...

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