Anti-VEGF Receptor 2抗体[EIC] (ab9530)

Detection of VEGFR-2 extracellular domain of human umbilical vein endothelial cells (HUVEC) using monoclonal antibody VEGFR-2  (bold) versus control conditions.

IHC image of VEGF Receptor 2 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9530, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunocytochemical analysis of human aortic endothelial cells, labelling VEGF Receptor 2 with ab9530 (green). Cells cultured until 70% confluent and fixed with 4% paraformaldehyde for 15 minutes at room temperature. They were then incubated with ab9530, diluted in PBS containing 5% normal goat serum and 0.01% Triton X-100 at 4°C overnight. DAPI used to stain nuclei. 

Immunostaining of myocardium section. The arrowheads indicate VEGFR-2 positive endothelial cells from myocardial capillaries.

ab9530 staining Human aortic endothelial cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/2000 in PBS + 0.1% Triton-X 100 + 5% Goat serum) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Green - VEGF Receptor 2, Red - alpha tubulin, Blue - nuclear marker (DAPI).

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Wholemount Immunohistochemical analysis of lung tissue from a 6 day postnatal mouse, labelling VEGF Receptor 2 with ab9530. ab9530 was diluted 1/500 in 3% BSA and 0.1% Triton 100 in PBS buffer, and incubated at 4°C for 12 hours. The secondary used was a polyclonal Alexa Fluor 488 Goat Anti-Mouse IgG at 1/1000.

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