- 数据表
- 具体的参考文献 (16)
- 实验方案
概述
- 产品名称Anti-VEGF Receptor 2抗体[EIC]
参阅全部 VEGF Receptor 2 一抗 - 描述小鼠单克隆抗体[EIC] to VEGF Receptor 2
- 特异性The monoclonal antibody will detect native human VEGFR-2 / KDR in ELISA experiments and on the surface of different human cell types. It recognizes the transmembrane KDR e.g. in HUVECs which normally is fully glycosylated (200kDa form).
- 经测试应用适用于:
ICC/IF, IHC-P, ELISA, IHC-Fr, IHC - Wholemount, Flow Cytmore details
不适用于: WB - 种属反应性与反应: Rat, Sheep, Human
- 免疫原
Recombinant fragment corresponding to Human VEGF Receptor 2 aa 20-757. Recombinant human soluble VEGF Receptor 2.
Database link: P35968 - 阳性对照
- HUVEC cells. IHC-P: FFPE human placenta.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液pH: 7.40
Constituent: PBS -
Concentration information loading... - 纯度Protein G purified
- 克隆单克隆
- 克隆编号EIC
- 同种型IgG1
- 研究领域
相关产品
- Compatible Secondaries
- Conjugation kits
- Immunohistochemistry kits
应用
Our Abpromise guarantee covers the use of ab9530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 16763549 | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ELISA | Use a concentration of 1 - 5 µg/ml. | |
| IHC-Fr | Use a concentration of 1 µg/ml. | |
| IHC - Wholemount | Use at an assay dependent concentration. | |
| Flow Cyt | Use a concentration of 2 - 10 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
- 功能Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
- 疾病相关Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
- 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. - 翻译后修饰Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
- 细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 3791 Human
- Entrez Gene: 25589 Rat
- Omim: 191306 Human
- SwissProt: P35968 Human
- SwissProt: O08775 Rat
- Unigene: 479756 Human
- Unigene: 88869 Rat
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别名
- CD309 antibody
- CD309 antigen antibody
- EC 2.7.10.1 antibody
see all
Anti-VEGF Receptor 2 antibody [EIC] 图像
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![Flow Cytometry - Anti-VEGF Receptor 2 antibody [EIC] (ab9530)](http://www.abcam.cn/ps/products/9/ab9530/Images/ab9530_2.gif)
Flow Cytometry - Anti-VEGF Receptor 2 antibody [EIC] (ab9530)Detection of VEGFR-2 extracellular domain of human umbilical vein endothelial cells (HUVEC) using monoclonal antibody VEGFR-2 (bold) versus control conditions.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody [EIC] (ab9530)](http://www.abcam.cn/ps/products/9/ab9530/Images/ab9530-233851-ab9530GR716694EDIT.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 2 antibody [EIC] (ab9530)IHC image of VEGF Receptor 2 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9530, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EIC] (ab9530)](http://www.abcam.cn/ps/products/9/ab9530/Images/ab9530-274280-ab9530-VEGFR2-ICC-3.png)
Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EIC] (ab9530)Image cropped from Czeisler and Mikawa, Public Library of Science, 9, e75833, Fig. 1a.; DOI: 10.1371/journal.pone.0075833Immunocytochemical analysis of human aortic endothelial cells, labelling VEGF Receptor 2 with ab9530 (green). Cells cultured until 70% confluent and fixed with 4% paraformaldehyde for 15 minutes at room temperature. They were then incubated with ab9530, diluted in PBS containing 5% normal goat serum and 0.01% Triton X-100 at 4°C overnight. DAPI used to stain nuclei.
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![Immunohistochemistry - Anti-VEGF Receptor 2 antibody [EIC] (ab9530)](http://www.abcam.cn/ps/products/9/ab9530/Images/ab9530_1.jpg)
Immunohistochemistry - Anti-VEGF Receptor 2 antibody [EIC] (ab9530)Immunostaining of myocardium section. The arrowheads indicate VEGFR-2 positive endothelial cells from myocardial capillaries.
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![Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EIC] (ab9530)](http://www.abcam.cn/ps/products/9/ab9530/Images/ab9530-209099-ab9530icc.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EIC] (ab9530)This image is courtesy of an anonymous Abreview.ab9530 staining Human aortic endothelial cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/2000 in PBS + 0.1% Triton-X 100 + 5% Goat serum) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Green - VEGF Receptor 2, Red - alpha tubulin, Blue - nuclear marker (DAPI).
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![IHC - Wholemount - Anti-VEGF Receptor 2 antibody [EIC] (ab9530)](http://www.abcam.cn/ps/products/9/ab9530/Images/ab9530-274278-ab9530-VEGFR2-IHC-2.png)
IHC - Wholemount - Anti-VEGF Receptor 2 antibody [EIC] (ab9530)This image is courtesy of an anonymous Abreview.Wholemount Immunohistochemical analysis of lung tissue from a 6 day postnatal mouse, labelling VEGF Receptor 2 with ab9530. ab9530 was diluted 1/500 in 3% BSA and 0.1% Triton 100 in PBS buffer, and incubated at 4°C for 12 hours. The secondary used was a polyclonal Alexa Fluor 488 Goat Anti-Mouse IgG at 1/1000.
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![Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EIC] (ab9530)](http://www.abcam.cn/ps/products/9/ab9530/Images/VEGF-Receptor-2-Primary-antibodies-ab9530-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 2 antibody [EIC] (ab9530)This image is courtesy of an Abreview submitted by Jin-Seok Leeab9530 staining rat lung cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde and blocked in 5% normal goat serum for 1 hour at room temperature. Samples were incubated with primary antibody (dilution 1/50) for 14 hours at 4°C; the diution buffer was 5% NGS in 1 X TBS-T (0.1%tween20). A Cy3® -conjugated Goat polyclonal to mouse IgG, dilution 1/100, was used as secondary antibody.
Anti-VEGF Receptor 2 antibody [EIC] (ab9530)参考文献
This product has been referenced in:
- Basagiannis D et al. VEGF induces signalling and angiogenesis by directing VEGFR2 internalisation through macropinocytosis. J Cell Sci 129:4091-4104 (2016). Read more (PubMed: 27656109) »
- Lu Y et al. Expression of semaphorin 6D and its receptor plexin-A1 in gastric cancer and their association with tumor angiogenesis. Oncol Lett 12:3967-3974 (2016). WB, IHC-P ; Human . Read more (PubMed: 27895757) »
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