Anti-VEGF Receptor 2抗体(ab39638)


  • 产品名称Anti-VEGF Receptor 2抗体
    参阅全部 VEGF Receptor 2 一抗
  • 描述
    兔多克隆抗体to VEGF Receptor 2
  • 特异性This antibody detects endogenous levels of total VEGF Receptor 2 protein.
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    The antiserum was produced against synthesized non-phosphopeptide derived from human VEGF Receptor 2 around the phosphorylation site of tyrosine 951.



Our Abpromise guarantee covers the use of ab39638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 151 kDa.
ELISA 1/20000.
IHC-P 1/50 - 1/100.


  • 功能Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • 疾病相关Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD309 antibody
    • CD309 antigen antibody
    • EC antibody
    • Fetal liver kinase 1 antibody
    • FLK-1 antibody
    • FLK1 antibody
    • FLK1, mouse, homolog of antibody
    • Kdr antibody
    • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
    • Kinase insert domain receptor antibody
    • KRD1 antibody
    • Ly73 antibody
    • Protein tyrosine kinase receptor FLK1 antibody
    • Protein-tyrosine kinase receptor flk-1 antibody
    • soluble VEGFR2 antibody
    • Tyrosine kinase growth factor receptor antibody
    • Vascular endothelial growth factor receptor 2 antibody
    • VEGFR 2 antibody
    • VEGFR antibody
    • VEGFR-2 antibody
    • VEGFR2 antibody
    • VGFR2_HUMAN antibody
    see all

Anti-VEGF Receptor 2 antibody 图像

  • Predicted band size : 151 kDa

    Western blot analysis of extracts from SK-OV3 cells using VEGF Receptor 2 antibody ab39638 (Lane 1 and 2) and VEGF Receptor 2 (phospho-Tyr951) antibody (Lane 3 and 4).


    Secondary antibody - anti-rabbit HRP (ab6721)

  • Ab39638 staining human VEGF Receptor 2 in human breast carcinoma by immunohistochemistry using paraffin embedded tissue.
  • ICC/IF image of ab39638 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39638, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-VEGF Receptor 2 antibody (ab39638)参考文献

This product has been referenced in:
  • Lv J  et al. The chinese herbal decoction danggui buxue tang inhibits angiogenesis in a rat model of liver fibrosis. Evid Based Complement Alternat Med 2012:284963 (2012). Rat . Read more (PubMed: 22927877) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Heart, with a myocardial infarction)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization No
Specification Heart, with a myocardial infarction
Blocking step 10% goat serum and 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Formaldehyde

Anita Chandler

Verified customer

提交于 Jul 28 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (human stomach cancer cell derived tissue)
Loading amount 30 µg
Specification human stomach cancer cell derived tissue
Treatment 20uM cannabinoid antagonist
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

提交于 Apr 09 2013

Thank you for your reply. That is super news. Would it be possible for you to submit these images to our datasheets via our Abreview system? We always encourage customers to send their results back to us, whether positive or negative, and we make all p...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. I look forward to receiving your images of ab39638. As requested, I have issued a free of charge replacement of ab9530. I am out of the off...

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Thank you for your enquiry. To our knowledge ab36993 has not been tested for reactivity to mouse LYVE1. It may cross react with mouse protein, however, as it is a polyclonal product raised against a full length immunogen protein. I hope...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab36993. To check the status of the order please contact our Customer...

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I am sorry to hear the replacement vial of ab39638 did not improve things. Again, I would be happy to offer a different primary antibody, credit or refund. Please let me know how you wish to proceed.

Thanks for your enquiry. I can only send out 1 vial of antibody to replace the 1 vial that was not working as expected. Please note as well that we have sold out of lot 762406 for ab39638. Please let me know if you need anything further.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab39638. To check the status of the order please contact our C...

Read More