概述

  • 产品名称Anti-VEGF Receptor 2抗体
    参阅全部 VEGF Receptor 2 一抗
  • 描述
    兔多克隆抗体to VEGF Receptor 2
  • 特异性The polyclonal antibody will detect native and recombinant human VEGFR2/KDR in ELISA experiments and on the surface or solubilized from different human cell types.
  • 经测试应用适用于: Sandwich ELISA, ELISA, WB, Flow Cyt, IHC-Fr, IP, Neutralisingmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment corresponding to Human VEGF Receptor 2. Highly pure recombinant human soluble extracellular domain of KDR (110 kDa) (D1-D7).

应用

Our Abpromise guarantee covers the use of ab11939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228).

For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab42228 as Detection.

ELISA Use a concentration of 5 - 15 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 151 kDa.
Flow Cyt Use a concentration of 1 - 5 µg/ml. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at 1-2 µg/mg of lysate.
Neutralising Use a concentration of 20 - 25 µg/ml. PubMed: 17991870

靶标

  • 功能Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • 疾病相关Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD309 antibody
    • CD309 antigen antibody
    • EC 2.7.10.1 antibody
    • Fetal liver kinase 1 antibody
    • FLK-1 antibody
    • FLK1 antibody
    • FLK1, mouse, homolog of antibody
    • Kdr antibody
    • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
    • Kinase insert domain receptor antibody
    • KRD1 antibody
    • Ly73 antibody
    • Protein tyrosine kinase receptor FLK1 antibody
    • Protein-tyrosine kinase receptor flk-1 antibody
    • soluble VEGFR2 antibody
    • Tyrosine kinase growth factor receptor antibody
    • Vascular endothelial growth factor receptor 2 antibody
    • VEGFR 2 antibody
    • VEGFR antibody
    • VEGFR-2 antibody
    • VEGFR2 antibody
    • VGFR2_HUMAN antibody
    see all

Anti-VEGF Receptor 2 antibody 图像

  • Flow Cytometry analysis of primary human dermal lymphatic endothelial cells (HDLEC) labelling VEGF Receptor 2 with ab11939.

  • Flow Cytometry analysis of primary human umbilical vein endothelial cells (HUVEC) labelling VEGF Receptor 2 with ab11939.

  • Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.

  • Immunohistochemistry (Frozen sections) analysis of human spleen tissue sections labelling VEGF Receptor 2 with ab11939.

  • Standard curve for VEGF Receptor 2 (Analyte: ab54347); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 (ab42228) at 5µg/ml and Detector Antibody Rabbit polyclonal to VEGF Receptor 2 (ab11939) at 0.5µg/ml.

实验方案

Anti-VEGF Receptor 2 antibody (ab11939)参考文献

This product has been referenced in:
  • Zhang L  et al. Transcription factor E2-2 inhibits the proliferation of endothelial progenitor cells by suppressing autophagy. Int J Mol Med N/A:N/A (2016). Flow Cyt ; Mouse . Read more (PubMed: 26986900) »
  • Xia X  et al. Inhibitor of DNA binding 1 regulates cell cycle progression of endothelial progenitor cells through induction of Wnt2 expression. Mol Med Rep N/A:N/A (2016). Read more (PubMed: 27432753) »

See all 6 Publications for this product

Product Wall

We have located the order associated with this purchase of ab11939 .It was purchased in May, 2008, which is well beyond the 6 month guarantee period we offer on all our products.

Is it possible that over the years, others in your lab have use...

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Thanks for following up with me regarding the PO number for ab11939.

Unfortunately I have not found this number to correspond to any orders in our system. Also I have searched our last 2 years of orders for ab11939 and not found any from UC R...

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Thank you for your email. Ab11939 has been tested for application in WB and IP with human samples, and our policy is that we guarantee that the product will work as stated on the online datasheet. If not, and the product has been purchased in the past ...

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Thank you for your email. Yes, ab23734 has been tested for application in both IP and Western blotting. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. For ab1193...

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