Anti-VEGF Receptor 2抗体(ab10972)

概述

  • 产品名称Anti-VEGF Receptor 2抗体
    参阅全部 VEGF Receptor 2 一抗
  • 描述
    山羊多克隆抗体to VEGF Receptor 2
  • 特异性Based on immunoblotting and ELISA, this antibody shows approximately 2% cross-reactivity with recombinant mouse VEGF Receptor 1, and no cross-reactivity with recombinant human VEGF Receptor 1 and VEGF Receptor 3.
  • 经测试应用适用于: ICC/IF, Indirect ELISA, IHC-P, Flow Cyt, ICC, WB, Neutralising, Blockingmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog
    不与反应: Human
  • 免疫原

    Recombinant fragment from the extracellular domain (Mouse) expressed in mouse NSO cells.

  • 常规说明Do not store in frost-free freezer. Endotoxin level is < 10 ng/mg antibody as determined by the LAL (Limulus amebocyte lysate) method.

应用

Our Abpromise guarantee covers the use of ab10972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 - 15 µg/ml. (and tissues).
Indirect ELISA Use a concentration of 0.5 - 1 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 20882677
Flow Cyt Use a concentration of 3 - 10 µg/ml.

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration. See Abreview.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 152 kDa.
Neutralising Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration. PubMed: 20884890

靶标

  • 功能Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • 疾病相关Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD309 antibody
    • CD309 antigen antibody
    • EC 2.7.10.1 antibody
    • Fetal liver kinase 1 antibody
    • FLK-1 antibody
    • FLK1 antibody
    • FLK1, mouse, homolog of antibody
    • Kdr antibody
    • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
    • Kinase insert domain receptor antibody
    • KRD1 antibody
    • Ly73 antibody
    • Protein tyrosine kinase receptor FLK1 antibody
    • Protein-tyrosine kinase receptor flk-1 antibody
    • soluble VEGFR2 antibody
    • Tyrosine kinase growth factor receptor antibody
    • Vascular endothelial growth factor receptor 2 antibody
    • VEGFR 2 antibody
    • VEGFR antibody
    • VEGFR-2 antibody
    • VEGFR2 antibody
    • VGFR2_HUMAN antibody
    see all

Anti-VEGF Receptor 2 antibody 图像

  • Ab10972 positively staining VEGF receptor 2 in paraformaldehyde fixed mouse retinal pigment epithelium (10 µg/ml). Used in conjunction with donkey anti goat IgG (Alexa ® 488) at 1/100.

    This image is courtesy of an Abreview submitted on 12th October 2005. We do not have any further information relating to this image.

Anti-VEGF Receptor 2 antibody (ab10972)参考文献

This product has been referenced in:
  • Wang TC  et al. Characterization of highly proliferative secondary tumor clusters along host blood vessels in malignant glioma. Mol Med Rep 12:6435-44 (2015). IHC-Fr ; Rat . Read more (PubMed: 26299849) »
  • Soda Y  et al. From the Cover: Feature Article: Transdifferentiation of glioblastoma cells into vascular endothelial cells. Proc Natl Acad Sci U S A 108:4274-80 (2011). IHC-P ; Mouse . Read more (PubMed: 21262804) »

See all 7 Publications for this product

Product Wall

Thank you for contacting us. I ran a search in our catalogue according to the parameters you requested and came up with a list of about 37 antibodies (attached).  I don't think that looking for MHC Class antibodies will help since these are probably li...

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Thank you for your reply. I have now returned to the office an hope I can help further with your enquiry.

I can now confirm that of the antibodies we have directed against VEGF Receptor 2 (that are not raised in rabbit), most likely to react w...

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Reviewing the correspondence on this enquiry so far, I notice xxxxx has selected some antibodies not raised in rabbit and has been in contact with the originators to obtain further information regarding the immunogen sequence so we can work out wh...

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Thank you for contacting us this morning in regards to the anti-VEGF Receptor 2 antibody ab10972 and ab9530. I am sorry for the misunderstanding there seems to have been in this case.

As discussed over the phone, I am currently attempting to c...

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The datasheet does not state that the antibody does not work with human samples so I am sorry this actually voids the guarantee (http://www.abcam.com/index.html?pageconfig=abpromise) for replacement.

I wou...

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am at...

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We recommend ab96917, a rabbit anti-goat IgG secondary antibody that is pre-adsorbed to remove reactivity with rat IgG, among other species, and is conjugated to DyLight® 594.

Click here (or use the fo...

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Thank you for contacting us. I apologise for the delay and appreciate your patient and comprehension.

The immunogen for ab10972 and ab10975 consists of recombinant VEGF2-R extracellular domain. For ab10972 the immunogen was expressed in mouse...

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I am very pleased to hear you would like to accept our offer and testab10972 in IHC whole mount. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC whole mount and include...

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Application Western blot
Sample Human Cell lysate - whole cell (A549)
Loading amount 20 µg
Specification A549
Treatment TRAIL
Gel Running Conditions Reduced Denaturing (12.5% SDS-PAGE)
Blocking step (agent) for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
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提交于 Apr 02 2012

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