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Recombinant human soluble extracellular Flt-1 Ig-like loop 1 to 5 (sFlt-1(D5)).
VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity. Because sFLT1 has a higher affinity for VEGF than does FLK1, it may function as an inhibitor of VEGF response.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab32152 as a replacement.
Our Abpromise guarantee covers the use of ab9540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 1 - 10 µg/ml.|
|WB||Use a concentration of 2 - 5 µg/ml.|
|IHC-P||Use at an assay dependent concentration. PubMed: 17823277|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 25223735|
Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of Human Placenta, Placental Bed and Decidua Parietalis. VEGF Receptor 1 (geen) was labelled with ab9540 and VEGF Receptor 2 (red) was labelled with ab2349. Yellow - where both VEGF Receptor 1 and VEGF Receptor 2 are expressed. DAPI was used for nuclei staining (blue).
Immunofluorecent staining of human VEGF Receptor 1 in a co-culture of PAE-VEGF Receptor 1, PAE-KDR, and PAE-FLT-4 using ab9540. Goat anti-mouse ALEXA Flour at 1/6000 dilution was used as the secondary antibody.
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