Anti-VEGF 164抗体(ab53465)
Key features and details
- Rabbit polyclonal to VEGF 164
- Suitable for: IHC-P, RIA, ELISA, WB
- Reacts with: Rat, Cow, Human, Pig
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-VEGF 164抗体 -
描述
兔多克隆抗体to VEGF 164 -
宿主
Rabbit -
特异性
ab53465 reacts with all human VEGF isoforms (121, 165, 189, and 206). No cross reaction is observed with PDGF, FGF-1, FGF-2, and TGFa. -
经测试应用
适用于: IHC-P, RIA, ELISA, WBmore details -
种属反应性
与反应: Rat, Cow, Human, Pig -
免疫原
Recombinant bovine VEGF 164
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
-
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53465于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
RIA |
Use at an assay dependent concentration.
|
|
ELISA |
Use at an assay dependent concentration. PubMed: 21441982
|
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 27 kDa.
|
说明 |
---|
IHC-P
Use at an assay dependent concentration. |
RIA
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. PubMed: 21441982 |
WB
Use at an assay dependent concentration. Predicted molecular weight: 27 kDa. |
靶标
-
功能
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
组织特异性
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland. -
疾病相关
Microvascular complications of diabetes 1 -
序列相似性
Belongs to the PDGF/VEGF growth factor family. -
细胞定位
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
-
数据库链接
- Entrez Gene: 7422 Human
- Entrez Gene: 83785 Rat
- Omim: 192240 Human
- SwissProt: P15691 Cow
- SwissProt: P15692 Human
- SwissProt: P49151 Pig
- SwissProt: P16612 Rat
- Unigene: 73793 Human
see all -
别名
- Vascular endothelial growth factor A antibody
- Vascular permeability factor antibody
- VEGF antibody
see all
图片
-
Anti-VEGF 164 antibody (ab53465) at 1/500 dilution + Human thymus tissue lysate - total protein (ab30146) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
Additional bands at: 70 kDa (possible dimer), 70 kDa (possible glycosylated form)
数据表及文件
-
Datasheet download
文献 (54)
ab53465 被引用在 54 文献中.
- Wang Y et al. Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 molecules. J Mater Sci Mater Med 33:19 (2022). PubMed: 35072831
- Sui T et al. Gambogic amide inhibits angiogenesis by suppressing VEGF/VEGFR2 in endothelial cells in a TrkA-independent manner. Pharm Biol 59:1566-1575 (2021). PubMed: 34767490
- Zhu L & Liu Z Serum from patients with hypertension promotes endothelial dysfunction to induce trophoblast invasion through the miR-27b-3p/ATPase plasma membrane Ca2+ transporting 1 axis. Mol Med Rep 23:N/A (2021). PubMed: 33760199
- Zou W et al. ASK1/p38-mediated NLRP3 inflammasome signaling pathway contributes to aberrant retinal angiogenesis in diabetic retinopathy. Int J Mol Med 47:732-740 (2021). PubMed: 33416127
- Liang C et al. Downregulation of astrocyte elevated gene-1 expression inhibits the development of vasculogenic mimicry in gliomas. Exp Ther Med 21:22 (2021). PubMed: 33235631