Anti-VE Cadherin (phospho Y685)抗体(ab119785)


  • 产品名称
    Anti-VE Cadherin (phospho Y685)抗体
    参阅全部 VE Cadherin 一抗
  • 描述
    兔多克隆抗体to VE Cadherin (phospho Y685)
  • 特异性
    ab119785 detects a 140 kDa band corresponding to VE cadherin in western blots of Human endothelial cells treated with pervanadate, and this band is not detected in untreated cells or after alkaline phosphatase treatment.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phospho-peptide (Y685), conjugated to carrier protein, corresponding to amino acids surrounding tyrosine 685 in Human VE Cadherin.

  • 阳性对照
    • Human umbilical vein endothelial cells stimulated with pervanadate.
  • 常规说明
    Do not aliquot.



Our Abpromise guarantee covers the use of ab119785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 88 kDa).


  • 功能
    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.
  • 组织特异性
    Endothelial tissues and brain.
  • 序列相似性
    Contains 5 cadherin domains.
  • 翻译后修饰
    Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.
  • 细胞定位
    Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 7B 4 antibody
    • 7B4 antibody
    • 7B4 antigen antibody
    • CADH5_HUMAN antibody
    • Cadherin 5 antibody
    • Cadherin 5 type 2 antibody
    • Cadherin 5, type 2 (vascular endothelium) antibody
    • Cadherin 5, type 2, VE cadherin (vascular epithelium) antibody
    • cadherin, vascular endothelial antibody
    • cadherin, vascular endothelial, 1 antibody
    • Cadherin-5 antibody
    • Cadherin5 antibody
    • CD 144 antibody
    • CD144 antibody
    • CD144 antigen antibody
    • CDH 5 antibody
    • CDH5 antibody
    • CDH5 protein antibody
    • Endothelial specific cadherin antibody
    • FLJ17376 antibody
    • OTTHUMP00000174777 antibody
    • Vascular endothelial cadherin antibody
    • Vascular epithelium cadherin antibody
    • VE Cad antibody
    • VE-cadherin antibody
    • VEC antibody
    see all

Anti-VE Cadherin (phospho Y685) antibody 图像

  • All lanes : Anti-VE Cadherin (phospho Y685) antibody (ab119785) at 1/1000 dilution

    Lane 1 : Human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min.
    Lane 2 : Human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min then treated with alkaline phosphatase

    Predicted band size : 88 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)


Anti-VE Cadherin (phospho Y685) antibody (ab119785)参考文献

This product has been referenced in:
  • Lee CS  et al. Dipeptidyl Peptidase-4 Inhibitor Increases Vascular Leakage in Retina through VE-cadherin Phosphorylation. Sci Rep 6:29393 (2016). Read more (PubMed: 27381080) »
  • Zhang P  et al. Mutant B-Raf(V600E) Promotes Melanoma Paracellular Transmigration by Inducing Thrombin-mediated Endothelial Junction Breakdown. J Biol Chem 291:2087-106 (2016). Read more (PubMed: 26504080) »

See all 3 Publications for this product

Product Wall

Western blot
Mouse Tissue lysate - whole (lung)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
30 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Sep 09 2016

Thank you for your inquiry.

I am happy to confirm that both antibodies are tested and guaranteed for human sample in WB.

I would like to assure you that both antibodies are suitable for your purposes.

They are antibodies g...

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