Anti-VE Cadherin抗体- Intercellular Junction Marker (ab33168)

概述

  • 产品名称Anti-VE Cadherin抗体- Intercellular Junction Marker
    参阅全部 VE Cadherin 一抗
  • 描述
    兔多克隆抗体to VE Cadherin - Intercellular Junction Marker
  • 经测试应用适用于: ICC/IF, WB, IHC-Fr, IP, In-Cell ELISA, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Chicken, Human
    预测可用于: Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to Human VE Cadherin aa 750 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab27462)

  • 阳性对照
    • This antibody gave a positive signal in HUVEC (Human umbilical vein epithelial) Cell Lysate in Western blot, and in confluent HUVEC cells in ICC/IF.

性能

应用

Our Abpromise guarantee covers the use of ab33168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 5 µg/ml.

Abcam recommends using this product with confluent cells.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 87 kDa).Can be blocked with VE Cadherin peptide (ab27462).

Abcam recommends using BSA blocking with this product.  Milk blocking will give a greatly reduced signal strength in WB.

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
In-Cell ELISA Use at an assay dependent concentration. PubMed: 22689949
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.
  • 组织特异性Endothelial tissues and brain.
  • 序列相似性Contains 5 cadherin domains.
  • 翻译后修饰Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.
  • 细胞定位Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 7B 4 antibody
    • 7B4 antibody
    • 7B4 antigen antibody
    • CADH5_HUMAN antibody
    • Cadherin 5 antibody
    • Cadherin 5 type 2 antibody
    • Cadherin 5, type 2 (vascular endothelium) antibody
    • Cadherin 5, type 2, VE cadherin (vascular epithelium) antibody
    • cadherin, vascular endothelial antibody
    • cadherin, vascular endothelial, 1 antibody
    • Cadherin-5 antibody
    • Cadherin5 antibody
    • CD 144 antibody
    • CD144 antibody
    • CD144 antigen antibody
    • CDH 5 antibody
    • CDH5 antibody
    • CDH5 protein antibody
    • Endothelial specific cadherin antibody
    • FLJ17376 antibody
    • OTTHUMP00000174777 antibody
    • Vascular endothelial cadherin antibody
    • Vascular epithelium cadherin antibody
    • VE Cad antibody
    • VE-cadherin antibody
    • VEC antibody
    see all

Anti-VE Cadherin antibody - Intercellular Junction Marker 图像

  • Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) at 1 µg/ml + HUVEC Cell Lysate at 20 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 87 kDa
    Observed band size : 115 kDa (why is the actual band size different from the predicted?)
    The band we observe at 115 kDa is believed to be the glycosylated form of the protein.
  • ICC/IF image of VE-Cadherin staining on HUVEC cells using ab33168. The cells were incubated with the primary antibody (ab33168) and the secondary was FITC conjugated anti-rabbit used at 1:400. The cells were incubated with only the secondary antibody as a negative control.
  • ab33168 (1/50) staining VE Cadherin in paraffin-embbeded mouse heart tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer and blocking (5 minutes/peroxidase block and 10 minutes/protein block). For further experimental details please refer to abreview.

    See Abreview

  • ab33168 used in Flow cytometry.
    Human REH B cells were fixed in paraformaldehyde and permeabilized using saponin. Primary antibody used undiluted (2µl in 100µl of cells in PBS) and incubated for 15 minutes at 4°C. The secondary antibody used was an undiluted, Alexa Fluor®488 conjugated goat anti-rabbit IgG.

    Rabbit IgG isotype control (white)

    See Abreview

  • ab33168 Immunoprecipitating VE Cadherin in human HUVEC whole cell lysate. 1000000 cells lysate was incubated with primary antibody (1/100 in 0.5% NP40, 150mM NaCl, 50mM Tris) and matrix (Dynabeads) for 2 hours at 4°C. For western blotting a HRP-conjugated mouse anti-VE Cadherin (1/3000) was used to confirm successful immunoprecipation.

    See Abreview

  • ab33168 stained HUVEC cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33168 at 1µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • ICC/IF image of VE Cadherin stained HUVEC cells. The cells were incubated with the antibody ab33168 at 1/150 (Green). The cells were also stained with Rhodamine phalloidin (Red).

Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)参考文献

This product has been referenced in:
  • Ye X  et al. Altered ratios of pro- and anti-angiogenic VEGF-A variants and pericyte expression of DLL4 disrupt vascular maturation in infantile haemangioma. J Pathol 239:139-51 (2016). ICC/IF ; Human . Read more (PubMed: 26957058) »
  • Li C  et al. RhoA determines lineage fate of mesenchymal stem cells by modulating CTGF-VEGF complex in extracellular matrix. Nat Commun 7:11455 (2016). IHC (PFA fixed) . Read more (PubMed: 27126736) »

See all 37 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (primary rat asqtrocytes)
Permeabilization No
Specification primary rat asqtrocytes
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative Methanol
Username

Miss. lavinia capuana

Verified customer

提交于 Sep 23 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH9.0
Permeabilization No
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 08 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (lung endothelium)
Permeabilization Yes - Triton-X
Specification lung endothelium
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Methanol
Username

Veronika Winkelmann

Verified customer

提交于 Aug 04 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (bEND.3 and primary lung endothelial cells)
Permeabilization No
Specification bEND.3 and primary lung endothelial cells
Blocking step BSA in 0.1% PBST as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative Methanol
Username

Abcam user community

Verified customer

提交于 Jul 07 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Specification Liver
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 12 2016

Application Western blot
Sample Human Cell lysate - whole cell (HUVEC)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification HUVEC
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Feb 26 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse brain endothelial cell line bEnd.3)
Permeabilization Yes - 0.1% Triton in PBS
Specification Mouse brain endothelial cell line bEnd.3
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 27 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human Brain Microvascular Endothelial Cells)
Permeabilization Yes - 0.1% Triton in PBS
Specification Human Brain Microvascular Endothelial Cells
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 27 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Brain)
Permeabilization Yes - Triton x-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Miss. Tamara Martínez Valverde

Verified customer

提交于 Dec 07 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Sheep Tissue sections (Lung-Pulmonary Artery)
Permeabilization Yes - Triton-100
Specification Lung-Pulmonary Artery
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Acetone
Username

Christina

Verified customer

提交于 Nov 21 2015

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