Anti-VE Cadherin抗体(ab232880)
Key features and details
- Rabbit polyclonal to VE Cadherin
- Suitable for: WB, IHC-P
- Reacts with: Cow, Human
- Isotype: IgG
概述
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产品名称
Anti-VE Cadherin抗体
参阅全部 VE Cadherin 一抗 -
描述
兔多克隆抗体to VE Cadherin -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Cow, Human -
免疫原
Recombinant fragment (His-T7-tag) corresponding to Human VE Cadherin aa 50-400. Expressed in E.coli. N-terminal tags.
Database link: P33151 -
阳性对照
- IHC-P: Human lung, skin cancer, breast cancer, kidney, prostate and liver tissues. WB: Recombinant human VE cadherin protein; Cow kidney, brain and spleen lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232880于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 5 µg/ml.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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说明 |
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WB
Use a concentration of 1 - 5 µg/ml. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
靶标
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功能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. -
组织特异性
Endothelial tissues and brain. -
序列相似性
Contains 5 cadherin domains. -
翻译后修饰
Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. -
细胞定位
Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
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数据库链接
- Entrez Gene: 1003 Human
- Omim: 601120 Human
- SwissProt: P33151 Human
- Unigene: 76206 Human
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别名
- 7B 4 antibody
- 7B4 antibody
- 7B4 antigen antibody
see all
图片
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All lanes : Anti-VE Cadherin antibody (ab232880) at 2 µg/ml
Lane 1 : Cow kidney tissue lysate
Lane 2 : Cow brain tissue lysate
Lane 3 : Cow spleen tissue lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution -
Formalin-fixed, paraffin-embedded human kidney tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human breast cancer tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human skin cancer tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human lung tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human liver tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human prostate tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 µg/ml. DAB staining.
-
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human skin cancer tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 µg/ml. DAB staining.
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Formalin-fixed, paraffin-embedded human lung tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 µg/ml. DAB staining.
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Anti-VE Cadherin antibody (ab232880) at 2 µg/ml + Recombinant human VE cadherin protein
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab232880 被引用在 8 文献中.
- Cao J et al. Glaucocalyxin A alleviates lipopolysaccharide-induced inflammation and apoptosis in pulmonary microvascular endothelial cells and permeability injury by inhibiting STAT3 signaling. Exp Ther Med 23:313 (2022). PubMed: 35369532
- Li DK et al. Exosomal HMGA2 protein from EBV-positive NPC cells destroys vascular endothelial barriers and induces endothelial-to-mesenchymal transition to promote metastasis. Cancer Gene Ther 29:1439-1451 (2022). PubMed: 35388172
- Almahmoudi R et al. The effect of interleukin-17F on vasculogenic mimicry in oral tongue squamous cell carcinoma. Cancer Sci 112:2223-2232 (2021). PubMed: 33743555
- Hozain AE et al. Multiday maintenance of extracorporeal lungs using cross-circulation with conscious swine. J Thorac Cardiovasc Surg 159:1640-1653.e18 (2020). PubMed: 31761338
- Yan G et al. LncRNA MACC1-AS1 attenuates microvascular endothelial cell injury and promotes angiogenesis under hypoxic conditions via modulating miR-6867-5p/TWIST1 in human brain microvascular endothelial cells. Ann Transl Med 8:876 (2020). PubMed: 32793720
- Cai J et al. An In Vitro Bovine Cellular Model for Human Schlemm's Canal Endothelial Cells and Their Response to TGFß Treatment. Transl Vis Sci Technol 9:32 (2020). PubMed: 32832237
- Wu Q et al. Epithelial cell adhesion molecule and epithelial-mesenchymal transition are associated with vasculogenic mimicry, poor prognosis, and metastasis of triple negative breast cancer. Int J Clin Exp Pathol 12:1678-1689 (2019). PubMed: 31933986
- Cao S et al. The upregulation of miR-101 promotes vascular endothelial cell apoptosis and suppresses cell migration in acute coronary syndrome by targeting CDH5. Int J Clin Exp Pathol 12:3320-3328 (2019). PubMed: 31934175