The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 88 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.
组织特异性Endothelial tissues and brain.
序列相似性Contains 5 cadherin domains.
翻译后修饰Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.
细胞定位Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.